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Cytokeratin 18 antibody - VetSignal

Anti-Cytokeratin 18 antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134973
Anti-Cytokeratin 18 antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134973
Anti-Cytokeratin 18 antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134973
Anti-Cytokeratin 18 antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134973
Anti-Cytokeratin 18 antibody - VetSignal used in Western Blot (WB). GTX134973
Anti-Cytokeratin 18 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134973

Cat No. GTX134973

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Dog
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

MDCK

Predict Reactivity

Human, Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein corresponding to dog Cytokeratin 18.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

keratin 18,CK18

Database

Research Area

DATA IMAGES
Anti-Cytokeratin 18 antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134973

GTX134973 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog intestine.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX134973) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 18 antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134973

GTX134973 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog colon.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX134973) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 18 antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134973

GTX134973 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog liver.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX134973) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 18 antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134973

GTX134973 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog kidney.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX134973) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 18 antibody - VetSignal used in Western Blot (WB). GTX134973

GTX134973 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody (GTX134973) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Cytokeratin 18 antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134973

GTX134973 ICC/IF Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX134973) diluted at 1:500.

REFERENCE
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319