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Cytokeratin 18 (phospho Ser33) antibody

Anti-Cytokeratin 18 (phospho Ser33) antibody used in Western Blot (WB). GTX50318
Anti-Cytokeratin 18 (phospho Ser33) antibody used in IHC (Paraffin sections) (IHC-P). GTX50318

Cat No. GTX50318

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 33 (A-A-S(p)-V-Y) derived from human Cytokeratin 18.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

keratin 18 , CK-18 , CYK18 , K18

Cellular Localization

Nucleolus

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 18 (phospho Ser33) antibody used in Western Blot (WB). GTX50318

GTX50318 WB Image

WB analysis of extracts from HT-29 cells untreated or treated with EGF using GTX50318 Cytokeratin 18 (phospho Ser33) antibody.

Anti-Cytokeratin 18 (phospho Ser33) antibody used in IHC (Paraffin sections) (IHC-P). GTX50318

GTX50318 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50318 Cytokeratin 18 (phospho Ser33) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

There are currently no references for Cytokeratin 18 (phospho Ser33) antibody (GTX50318). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf