GeneTex
United States (US)

Cytokeratin 19 antibody [A53-B/A2]

Cytokeratin-19-antibody-A53-BA2__GTX27754-1_18121409_869.jpg

Cat No. GTX27754

Host Mouse
Clonality Monoclonal
Clone Name A53-B/A2
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IP, ELISA
Reactivity Human
APPLICATION

Application Note

For ELISA: Use at an assay dependent dilution. For ICC: Use at an assay dependent dilution. For IHC-P: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

44 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline, pH 7.4, containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human mammary carcinoma cell line MCF-7.

Purification

Protein A purified
Purified from hybridoma culture supernatant.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Keratin 19,Ck19,K19,K1Cs,Krt19

Cellular Localization

Cytoplasmic

Background

This protein is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis.

Database

Research Area

DATA IMAGES
Cytokeratin-19-antibody-A53-BA2__GTX27754-1_18121409_869.jpg

GTX27754 WB Image

Western Blot analysis (reducing conditions) of whole cell lysate of MCF-7 human breast adenocarcinoma cell line.
Lane 1,2: immunostaining with anti-human Cytokeratin 19 (BA-17) (GTX27755)
Lane 3,4: immunostaining with anti-human Cytokeratin 19 (A53-B/A2) (GTX27754)

REFERENCE
Package List Price ($)
$ 319