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Cytokeratin 19 antibody [A53-B/A2]

Orthogonal
Anti-Cytokeratin 19 antibody [A53-B/A2] used in Western Blot (WB). GTX27754
Anti-Cytokeratin 19 antibody [A53-B/A2] used in Western Blot (WB). GTX27754

Cat. No. GTX27754

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 A53-B/A2

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS, IP, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all Cytokeratin 19 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Product Note

This antibody reacts with Rod domain of cytokeratin 19 (40 kDa) in human tissue.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

MCF-7 human breast adenocarcinoma cell line

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_369535

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CK19 , K19 , K1CS , KRT19 , keratin 19 , Cytokeratin19 , CYFRA21-1 , Cyfra-21-1

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 19 antibody [A53-B/A2] used in Western Blot (WB). GTX27754

GTX27754 WB Image

WB analysis of MCF-7 cell lysate using GTX27754 Cytokeratin 19 antibody [A53-B/A2].
Lane 1,2 : GTX27755
Lane 3,4 : GTX27754

Anti-Cytokeratin 19 antibody [A53-B/A2] used in Western Blot (WB). GTX27754

GTX27754 WB Image

Orthogonal

The data was published in the journal Elife in 2016. PMID: 27003818

REFERENCE

Application Reference

Wu J et al. Elife 2016; 5 Polo-like kinase 1 induces epithelial-to-mesenchymal transition and promotes epithelial cell motility by activating CRAF/ERK signaling.
Application : WB
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399