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Cytokeratin 19 antibody [AT13D10]

Anti-Cytokeratin 19 antibody [AT13D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57591
Anti-Cytokeratin 19 antibody [AT13D10] used in Western Blot (WB). GTX57591
Anti-Cytokeratin 19 antibody [AT13D10] used in Western Blot (WB). GTX57591
Anti-Cytokeratin 19 antibody [AT13D10] used in Flow cytometry (FACS). GTX57591
Anti-Cytokeratin 19 antibody [AT13D10] used in Western Blot (WB). GTX57591

Cat. No. GTX57591

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT13D10

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($399)
See all Cytokeratin 19 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Recommended starting dilution is 1:1000.
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT13D10 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KRT21 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 19 , CK19 , K19 , K1CS , KRT19 , Cytokeratin19 , CYFRA21-1 , Cyfra-21-1

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 19 antibody [AT13D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57591

GTX57591 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX57591 Cytokeratin 19 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-Cytokeratin 19 antibody [AT13D10] used in Western Blot (WB). GTX57591

GTX57591 WB Image

WB analysis of various samples using GTX57591 Cytokeratin 19 antibody.
Lane 1 : 293T cell lysate
Lane 2 : KRT19 transfected 293T cell lysate
Loading : 5 μg
Dilution : 1:1000

Anti-Cytokeratin 19 antibody [AT13D10] used in Western Blot (WB). GTX57591

GTX57591 WB Image

WB analysis of MCF-7 whole cell lysate using GTX57591 Cytokeratin 19 antibody.
Loading : 40 μg
Dilution : 1:1000

Anti-Cytokeratin 19 antibody [AT13D10] used in Flow cytometry (FACS). GTX57591

GTX57591 FACS Image

FACS analysis of HepG2 cells using GTX57591 Cytokeratin 19 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

Anti-Cytokeratin 19 antibody [AT13D10] used in Western Blot (WB). GTX57591

GTX57591 WB Image

WB analysis of HepG2 whole cell lysate using GTX57591 Cytokeratin 19 antibody.
Loading : 20 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399