GeneTex
United States (US)

Cytokeratin 19 antibody [BA-17]

Cytokeratin-19-antibody-BA-17__GTX27755-1_18121409_206.jpg

Cat No. GTX27755

Host Mouse
Clonality Monoclonal
Clone Name BA-17
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS, IP
Reactivity Human, Mouse
APPLICATION

Application Note

For FACS: Use at an assay dependent dilution. For ICC: Use at an assay dependant dilution. For IHC-P: Use at an assay dependant dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. For IP: Use at an assay dependant dilution. For WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

44 kDa. ( Note )

Specificity/Sensitivity

Cytokeratin 19. Not expressed in hepatocytes.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4 containing 15mM Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Mammary organoids

Purification

Protein A affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Keratin 19,Ck19,K19,K1Cs,Krt19

Cellular Localization

Cytoplasmic

Background

This protein is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis.

Database

Research Area

DATA IMAGES
Cytokeratin-19-antibody-BA-17__GTX27755-1_18121409_206.jpg

GTX27755 WB Image

Detection of cytokeratin 19 in MCF-7 cell lysate by mouse monoclonal antibody BA-17 (GTX27755).

REFERENCE
Package List Price ($)
$ 319