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Cytokeratin 19 antibody [GT147]

Anti-Cytokeratin 19 antibody [GT147] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635163
Anti-Cytokeratin 19 antibody [GT147] used in Western Blot (WB). GTX635163

Cat. No. GTX635163

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT147

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 19 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cytokeratin 19. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888507

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

keratin 19 , CK19 , K19 , K1CS

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 19 antibody [GT147] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635163

GTX635163 ICC/IF Image

Cytokeratin 19 antibody [GT147] detects Cytokeratin 19 protein at cytoskeleton by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytokeratin 19 stained by Cytokeratin 19 antibody [GT147] (GTX635163) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-Cytokeratin 19 antibody [GT147] used in Western Blot (WB). GTX635163

GTX635163 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 19 antibody [GT147] (GTX635163) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations