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Cytokeratin 19 antibody [HL1165]

Recombinant antibody Orthogonal
Anti-Cytokeratin 19 antibody [HL1165] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636469
Anti-Cytokeratin 19 antibody [HL1165] used in IHC (Paraffin sections) (IHC-P). GTX636469
Anti-Cytokeratin 19 antibody [HL1165] used in IHC (Paraffin sections) (IHC-P). GTX636469
Anti-Cytokeratin 19 antibody [HL1165] used in Western Blot (WB). GTX636469

Cat. No. GTX636469

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1165

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 19 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cytokeratin 19. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909988

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 19 , CK19 , K19 , K1CS

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 19 antibody [HL1165] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636469

GTX636469 ICC/IF Image

Cytokeratin 19 antibody [HL1165] detects Cytokeratin 19 protein by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 10 min.
Green: Cytokeratin 19 stained by Cytokeratin 19 antibody [HL1165] (GTX636469) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-Cytokeratin 19 antibody [HL1165] used in IHC (Paraffin sections) (IHC-P). GTX636469

GTX636469 IHC-P Image

Cytokeratin 19 antibody [HL1165] detects Cytokeratin 19 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
Cytokeratin 19 stained by Cytokeratin 19 antibody [HL1165] (GTX636469) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 19 antibody [HL1165] used in IHC (Paraffin sections) (IHC-P). GTX636469

GTX636469 IHC-P Image

Cytokeratin 19 antibody [HL1165] detects Cytokeratin 19 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Cytokeratin 19 stained by Cytokeratin 19 antibody [HL1165] (GTX636469) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 19 antibody [HL1165] used in Western Blot (WB). GTX636469

GTX636469 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 19 antibody [HL1165] (GTX636469) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations