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Cytokeratin 19 antibody [N1C1]

Anti-Cytokeratin 19 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112826
Anti-Cytokeratin 19 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112826
Anti-Cytokeratin 19 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112826
Anti-Cytokeratin 19 antibody [N1C1] used in Western Blot (WB). GTX112826
Anti-Cytokeratin 19 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112826
Anti-Cytokeratin 19 antibody [N1C1] used in Western Blot (WB). GTX112826
Anti-Cytokeratin 19 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112826

Cat. No. GTX112826

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Dog
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 19 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 19. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950646

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 19 , CK19 , K19 , K1CS

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 19 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112826

GTX112826 IHC-P Image

Cytokeratin 19 antibody [N1C1] detects Cytokeratin 19 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tongue.
Cytokeratin 19 stained by Cytokeratin 19 antibody [N1C1] (GTX112826) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 19 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112826

GTX112826 IHC-P Image

Cytokeratin 19 antibody [N1C1] detects Cytokeratin 19 protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
Cytokeratin 19 antibody [N1C1] (GTX112826) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 19 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112826

GTX112826 IHC-P Image

Cytokeratin 19 antibody detects Cytokeratin 19 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog mammary gland.
Cytokeratin 19 stained by Cytokeratin 19 antibody (GTX112826) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 19 antibody [N1C1] used in Western Blot (WB). GTX112826

GTX112826 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 19 antibody (GTX112826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 19 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112826

GTX112826 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using Cytokeratin 19(GTX112826) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 19 antibody [N1C1] used in Western Blot (WB). GTX112826

GTX112826 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 19 antibody [N1C1] (GTX112826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 19 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112826

GTX112826 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 19 (GTX112826) antibody at 1:200 dilution.

REFERENCE

Application Reference

Moriggi M et al. Proteomics 2017; (Epub) Contribution of Extracellular Matrix and Signal Mechanotransduction to Epithelial Cell Damage in Inflammatory Bowel Disease Patients: A Proteomic Study.
Application : WB
Reactivity : Human
Chen Y et al. Hepatology 2012; 55 (2) : 563-74 Mature hepatocytes exhibit unexpected plasticity by direct dedifferentiation into liver progenitor cells in culture.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169