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Cytokeratin 19 antibody [N1C1]

Anti-Cytokeratin 19 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112826
Anti-Cytokeratin 19 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112826
Anti-Cytokeratin 19 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112826
Anti-Cytokeratin 19 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112826
Anti-Cytokeratin 19 antibody [N1C1] used in Western Blot (WB). GTX112826
Anti-Cytokeratin 19 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112826
Anti-Cytokeratin 19 antibody [N1C1] used in Western Blot (WB). GTX112826

Cat. No. GTX112826

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Dog
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 19 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 19. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950646

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

keratin 19 , CK19 , K19 , K1CS

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 19 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112826

GTX112826 IHC-P Image

Cytokeratin 19 antibody [N1C1] detects Cytokeratin 19 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tongue.
Cytokeratin 19 stained by Cytokeratin 19 antibody [N1C1] (GTX112826) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 19 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112826

GTX112826 IHC-P Image

Cytokeratin 19 antibody [N1C1] detects Cytokeratin 19 protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
Cytokeratin 19 antibody [N1C1] (GTX112826) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 19 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112826

GTX112826 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 19 (GTX112826) antibody at 1:200 dilution.

Anti-Cytokeratin 19 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112826

GTX112826 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using Cytokeratin 19(GTX112826) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 19 antibody [N1C1] used in Western Blot (WB). GTX112826

GTX112826 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 19 antibody [N1C1] (GTX112826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 19 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112826

GTX112826 IHC-P Image

Cytokeratin 19 antibody detects Cytokeratin 19 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog mammary gland.
Cytokeratin 19 stained by Cytokeratin 19 antibody (GTX112826) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 19 antibody [N1C1] used in Western Blot (WB). GTX112826

GTX112826 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 19 antibody (GTX112826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Moriggi M et al. Proteomics 2017; (Epub) Contribution of Extracellular Matrix and Signal Mechanotransduction to Epithelial Cell Damage in Inflammatory Bowel Disease Patients: A Proteomic Study.
Application : WB
Reactivity : Human
Chen Y et al. Hepatology 2012; 55 (2) : 563-74 Mature hepatocytes exhibit unexpected plasticity by direct dedifferentiation into liver progenitor cells in culture.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169