Cytokeratin 19 antibody [SPM266]

Cat. No. GTX34648

Host	Mouse
Clonality	Monoclonal
Clone Name	SPM266
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μg ($529)

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Datasheet
Document

View product citations for antibody GTX34648 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
ICC/IF	1-2μg/ml
IHC-P	1-2μg/ml for 30 min at RT
FACS	1-2μg/10⁶ cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Product Note

This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. Its epitope maps between amino acid 312-335.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human breast cancer MCF-7 cells

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CK19 , K19 , K1CS , KRT19 , keratin 19 , Cytokeratin19 , CYFRA21-1 , Cyfra-21-1

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Database

Gene ID: 3880 KRT19
UniProt: P08727 KRT19

Research Area

DATA IMAGES

GTX34648 IHC-P Image

IHC-P analysis of human endometrial carcinoma tissue using GTX34648 Cytokeratin 19 antibody [SPM266].

GTX34648 FACS Image

FACS analysis of Methanol-fixed MCF-7 cells using GTX34648 Cytokeratin 19 antibody [SPM266].
Blue : Primary antibody
Red : Isotype control

GTX34648 WB Image

WB analysis of HepG2 cell lysate using GTX34648 Cytokeratin 19 antibody [SPM266].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty