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Cytokeratin 19 antibody [SPM266]

Anti-Cytokeratin 19 antibody [SPM266] used in Western Blot (WB). GTX34648
Anti-Cytokeratin 19 antibody [SPM266] used in IHC (Paraffin sections) (IHC-P). GTX34648
Anti-Cytokeratin 19 antibody [SPM266] used in Flow cytometry (FACS). GTX34648

Cat No. GTX34648

Host

Mouse

Clonality

Monoclonal

Clone Name

SPM266

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Specificity/Sensitivity

This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. Its epitope maps between amino acid 312-335.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human breast cancer MCF-7 cells

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CK19 , K19 , K1CS , KRT19 , keratin 19 , Cytokeratin19 , CYFRA21-1 , Cyfra-21-1

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 19 antibody [SPM266] used in Western Blot (WB). GTX34648

GTX34648 WB Image

WB analysis of HepG2 cell lysate using GTX34648 Cytokeratin 19 antibody [SPM266].

Anti-Cytokeratin 19 antibody [SPM266] used in IHC (Paraffin sections) (IHC-P). GTX34648

GTX34648 IHC-P Image

IHC-P analysis of human endometrial carcinoma tissue using GTX34648 Cytokeratin 19 antibody [SPM266].

Anti-Cytokeratin 19 antibody [SPM266] used in Flow cytometry (FACS). GTX34648

GTX34648 FACS Image

FACS analysis of Methanol-fixed MCF-7 cells using GTX34648 Cytokeratin 19 antibody [SPM266].
Blue : Primary antibody
Red : Isotype control

REFERENCE

There are currently no references for Cytokeratin 19 antibody [SPM266] (GTX34648). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339