APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2μg/ml |
1-2μg/ml |
1-2μg/ml for 30 min at RT |
1-2μg/10⁶ cells |
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
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Not tested in other applications.
Calculated MW
Product Note
This Ab reacts with the rod domain of human cytokeratin 19 (CK19), a polypeptide of 40kDa.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human mammary epithelial organoids.
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CK19 , K19 , K1CS , KRT19 , keratin 19 , Cytokeratin19 , CYFRA21-1 , Cyfra-21-1
Background
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX34649 FACS Image
FACS analysis of MeOH-fixed MCF-7 cells using GTX34649 Cytokeratin 19 antibody [SPM561]. Blue : Primary antibody Red : Isotype control
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GTX34649 WB Image
WB analysis of HepG2 cell lysate using GTX34649 Cytokeratin 19 antibody [SPM561].
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GTX34649 IHC-P Image
IHC-P analysis of human bladder carcinoma tissue using GTX34649 Cytokeratin 19 antibody [SPM561].
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GTX34649 ICC/IF Image
ICC/IF analysis of MeOH-fixed MCF-7 cells using GTX34649 Cytokeratin 19 antibody [SPM561]. Green : Primary antibody Red : nucleus
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REFERENCE
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REVIEW
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