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Cytokeratin 2 antibody [KRTH/2147R]

Anti-Cytokeratin 2 antibody [KRTH/2147R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02662
Anti-Cytokeratin 2 antibody [KRTH/2147R] used in IHC (Paraffin sections) (IHC-P). GTX02662

Cat. No. GTX02662

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 KRTH/2147R

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($529)
See all Cytokeratin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/ml
ICC/IF 1-2 μg/ml
IHC-P 0.25-0.5 μg/ml
FACS 1-2 μg/million cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Observed MW

50-67 kDa.

Product Note

This antibody recognizes CK1, CK5, CK10 and CK14. It shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, endometrial glands, or with cells derived from simple epithelia.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Solubilized keratin extract from human stratum corneum

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 76 , HUMCYT2A , KRT2B , KRT2P

Background

Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13. [provided by RefSeq, Jun 2009]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 2 antibody [KRTH/2147R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02662

GTX02662 ICC/IF Image

ICC/IF analysis of MeOH-fixed MCF-7 cells using GTX02662 Cytokeratin 2 antibody [KRTH/2147R].
Green : Primary antibody
Red : nuclear counterstain

Anti-Cytokeratin 2 antibody [KRTH/2147R] used in IHC (Paraffin sections) (IHC-P). GTX02662

GTX02662 IHC-P Image

IHC-P analysis of human prostate carcinoma section using GTX02662 Cytokeratin 2 antibody [KRTH/2147R].

REFERENCE

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REVIEW

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Package List Price ($)
$ 529