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Cytokeratin 2 antibody [N2C2], Internal

Anti-Cytokeratin 2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102758
Anti-Cytokeratin 2 antibody [N2C2], Internal used in Western Blot (WB). GTX102758
Anti-Cytokeratin 2 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102758
Anti-Cytokeratin 2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102758
Anti-Cytokeratin 2 antibody [N2C2], Internal used in Western Blot (WB). GTX102758

Cat. No. GTX102758

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Dog
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950648

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 2 , CK-2e , K2e , KRT2A , KRT2E , KRTE

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102758

GTX102758 ICC/IF Image

Cytokeratin 2 antibody detects Cytokeratin 2 protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytokeratin 2 stained by Cytokeratin 2 antibody (GTX102758) diluted at 1:500.

Anti-Cytokeratin 2 antibody [N2C2], Internal used in Western Blot (WB). GTX102758

GTX102758 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 2 antibody (GTX102758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 2 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102758

GTX102758 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using KRT2(GTX102758) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102758

GTX102758 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cytokeratin 2(GTX102758) antibody at 1:200 dilution.

Anti-Cytokeratin 2 antibody [N2C2], Internal used in Western Blot (WB). GTX102758

GTX102758 WB Image

A431 cell extract (A, 30 μg) was separated by 10% SDS-PAGE, and blotted with Cytokeratin 2 antibody [N2C2], Internal (GTX102758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Liu JF et al. Mol Carcinog 2017; (Epub) Inhibition of JAK2/STAT3 reduces tumor-induced angiogenesis and myeloid-derived suppressor cells in head and neck cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169