GeneTex
United States (US)

Cytokeratin 2 antibody [N2C2], Internal

GTX102758_WB_19011506_260.jpg
GTX102758_39883_IFA_19011506_938.jpg
GTX102758_IHC_19011506_102.jpg

Cat No. GTX102758

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Dog, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Keratin 2,Ck-2E,K2E,Krt2A,Krt2E,Krte,Krt2

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102758_WB_19011506_260.jpg

GTX102758 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)

GTX102758_39883_IFA_19011506_938.jpg

GTX102758 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cytokeratin 2(GTX102758) antibody at 1:200 dilution.

GTX102758_IHC_19011506_102.jpg

GTX102758 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using KRT2(GTX102758) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169