A431 cell extract (A, 30 μg) was separated by 10% SDS-PAGE, and blotted with Cytokeratin 2 antibody [N2C2], Internal (GTX102758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cytokeratin 2(GTX102758) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using KRT2(GTX102758) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 2 antibody (GTX102758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cytokeratin 2 antibody detects Cytokeratin 2 protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytokeratin 2 stained by Cytokeratin 2 antibody (GTX102758) diluted at 1:500.