GeneTex
United States (US)

Cytokeratin 20 antibody [AT10D8]

GTX16500_FACS_18121410_338.jpg
GTX16500_WB_18121410_758.jpg
GTX16500_ICCIF_18121410_945.jpg

Cat No. GTX16500

Host Mouse
Clonality Monoclonal
Clone Name AT10D8
Isotype IgG2a
Application WB, ICC/IF, FACS, ELISA
Reactivity Human
APPLICATION

Application Note

WB: 1:3000. FACS: 1:200

Calculated MW

48 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human KRT20 (1-424aa)

Purification

By protein-A affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Keratin 20,Cd20,Ck-20,Ck20,K20,Krt21,Krt20

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX16500_FACS_18121410_338.jpg

GTX16500 FACS Image

FACS analysis of A549 cells using an isotype control (black) or Cytokeratin 20 antibody [AT10D8] at 2-5ug for 1,000,000 cells (red).

GTX16500_WB_18121410_758.jpg

GTX16500 WB Image

WB analysis of (1) recombinant human Cytokeratin 20 (25ng), (2) HeLa and (3) A431 lysates (40ug of lysates) using Cytokeratin 20 antibody [AT10D8] at a dilution of 1:3000.

GTX16500_ICCIF_18121410_945.jpg

GTX16500 ICC/IF Image

ICC/IF analysis of A549 cells using DAPI (blue) and Cytokeratin 20 antibody [AT10D8] at a dilution of 1:200 (green).

Package List Price ($)
$ 319