Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 20 antibody [C2C3], C-term (GTX100693) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded HT29 xenograft , using Cytokeratin 20(GTX100693) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min