GeneTex
United States (US)

Cytokeratin 20 antibody [C2C3], C-term

GTX100693_IHC_19011506_176.jpg
GTX100693_WB_19011506_350.jpg

Cat No. GTX100693

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLaS3

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cytokeratin 20. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Keratin 20,Cd20,Ck-20,Ck20,K20,Krt21,Krt20

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100693_IHC_19011506_176.jpg

GTX100693 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HT29 xenograft , using Cytokeratin 20(GTX100693) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100693_WB_19011506_350.jpg

GTX100693 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
10% SDS PAGE
GTX100693 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169