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Cytokeratin 20 antibody [KRT20/1992]

Anti-Cytokeratin 20 antibody [KRT20/1992] used in Protein Array (Protein Array). GTX17981
Anti-Cytokeratin 20 antibody [KRT20/1992] used in IHC (Paraffin sections) (IHC-P). GTX17981
Anti-Cytokeratin 20 antibody [KRT20/1992] used in IHC (Paraffin sections) (IHC-P). GTX17981
Anti-Cytokeratin 20 antibody [KRT20/1992] used in Western Blot (WB). GTX17981

Cat. No. GTX17981

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 KRT20/1992

Isotype

 IgG2b

Application

 WB, IHC-P, Protein Array

Reactivity

 Human
Package
100 μg ($529)
See all Cytokeratin 20 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5μg/ml-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD20 , CK20 , K20 , KRT20 , KRT21 , keratin 20 , Cytokeratin 20

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 20 antibody [KRT20/1992] used in Protein Array (Protein Array). GTX17981

GTX17981 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (KRT20/1992). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-Cytokeratin 20 antibody [KRT20/1992] used in IHC (Paraffin sections) (IHC-P). GTX17981

GTX17981 IHC-P Image

IHC-P analysis of human colon tissue using GTX17981 Cytokeratin 20 antibody [KRT20/1992].

Anti-Cytokeratin 20 antibody [KRT20/1992] used in IHC (Paraffin sections) (IHC-P). GTX17981

GTX17981 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX17981 Cytokeratin 20 antibody [KRT20/1992].

Anti-Cytokeratin 20 antibody [KRT20/1992] used in Western Blot (WB). GTX17981

GTX17981 WB Image

WB analysis of HT29 cell lysate using GTX17981 Cytokeratin 20 antibody [KRT20/1992].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529