Cytokeratin 20 antibody [N2C2], Internal

Cytokeratin 20 antibody [N2C2], Internal detects Cytokeratin 20 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Cytokeratin 20 stained by Cytokeratin 20 antibody [N2C2], Internal (GTX110600) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 20 antibody [N2C2], Internal (GTX110600) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded HT29 xenograft, using Cytokeratin 20(GTX110600) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 20(GTX110600) antibody at 1:200 dilution.

The data was published in the journal Proteome Sci in 2014. PMID: 25075203