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Cytokeratin 20 antibody [N2C2], Internal

Orthogonal
Anti-Cytokeratin 20 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110600
Anti-Cytokeratin 20 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110600
Anti-Cytokeratin 20 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110600
Anti-Cytokeratin 20 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110600
Anti-Cytokeratin 20 antibody [N2C2], Internal used in Western Blot (WB). GTX110600
Anti-Cytokeratin 20 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110600

Cat. No. GTX110600

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 20 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 20. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950649

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 20 , CD20 , CK-20 , CK20 , K20 , KRT21

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 20 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110600

GTX110600 IHC-P Image

Cytokeratin 20 antibody [N2C2], Internal detects Cytokeratin 20 protein at cytoplasm on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
Cytokeratin 20 antibody [N2C2], Internal (GTX110600) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 20 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110600

GTX110600 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 20(GTX110600) antibody at 1:200 dilution.

Anti-Cytokeratin 20 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110600

GTX110600 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HT29 xenograft, using Cytokeratin 20(GTX110600) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 20 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110600

GTX110600 IHC-P Image

Cytokeratin 20 antibody [N2C2], Internal detects Cytokeratin 20 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Cytokeratin 20 stained by Cytokeratin 20 antibody [N2C2], Internal (GTX110600) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 20 antibody [N2C2], Internal used in Western Blot (WB). GTX110600

GTX110600 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 20 antibody [N2C2], Internal (GTX110600) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Cytokeratin 20 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110600

GTX110600 IHC-P Image

The data was published in the journal Proteome Sci in 2014. PMID: 25075203

REFERENCE

Application Reference

Fan Y et al. Cell Death Differ 2022; Cullin 4b-RING ubiquitin ligase targets IRGM1 to regulate Wnt signaling and intestinal homeostasis.
Application : WB
Reactivity : Mouse
Yen YT et al. Cells 2020; 9 (1) : pii: E90 PP2A Deficiency Enhances Carcinogenesis of Lgr5+ Intestinal Stem Cells Both in Organoids and In Vivo.
Application : IHC-P
Reactivity : Mouse
Yen YT et al. Cells 2019; 9 (1) PP2A Deficiency Enhances Carcinogenesis of Lgr5+ Intestinal Stem Cells Both in Organoids and In Vivo.
Application : ICC/IF
Reactivity : Mouse
Usami S et al. J Plast Surg Hand Surg 2016; (Epub) Histological investigation of common insensate flaps obtained from the hand and forearm regions for use in fingertip reconstruction.
Rajcevic U et al. Proteome Sci 2014; 12 : 39 Colorectal cancer derived organotypic spheroids maintain essential tissue characteristics but adapt their metabolism in culture.
Application : IHC-P
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169