Cytokeratin 20 antibody [N2C2], Internal

Name: Cytokeratin 20 antibody [N2C2], Internal
Brand: GeneTex
SKU: GTX110600
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Cytokeratin 20 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110600

Anti-Cytokeratin 20 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110600

Anti-Cytokeratin 20 antibody [N2C2], Internal used in Western Blot (WB). GTX110600

Cat. No. GTX110600

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all Cytokeratin 20 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 20. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950649

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 20 , CD20 , CK-20 , CK20 , K20 , KRT21

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq]

Database

Gene ID: 54474 KRT20
UniProt: P35900 KRT20

Research Area

DATA IMAGES

GTX110600 IHC-P Image

Cytokeratin 20 antibody [N2C2], Internal detects Cytokeratin 20 protein at cytoplasm on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
Cytokeratin 20 antibody [N2C2], Internal (GTX110600) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX110600 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 20(GTX110600) antibody at 1:200 dilution.

GTX110600 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HT29 xenograft, using Cytokeratin 20(GTX110600) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX110600 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 20 antibody [N2C2], Internal (GTX110600) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Fan Y et al. Cell Death Differ 2022; Cullin 4b-RING ubiquitin ligase targets IRGM1 to regulate Wnt signaling and intestinal homeostasis.

Application : WB

Reactivity : Mouse

Yen YT et al. Cells 2020; 9 (1) : pii: E90 PP2A Deficiency Enhances Carcinogenesis of Lgr5+ Intestinal Stem Cells Both in Organoids and In Vivo.

Application : IHC-P

Reactivity : Mouse

Yen YT et al. Cells 2019; 9 (1) PP2A Deficiency Enhances Carcinogenesis of Lgr5⁺ Intestinal Stem Cells Both in Organoids and In Vivo.

Application : ICC/IF

Reactivity : Mouse

Usami S et al. J Plast Surg Hand Surg 2016; (Epub) Histological investigation of common insensate flaps obtained from the hand and forearm regions for use in fingertip reconstruction.

Rajcevic U et al. Proteome Sci 2014; 12 : 39 Colorectal cancer derived organotypic spheroids maintain essential tissue characteristics but adapt their metabolism in culture.

Application : IHC-P

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