Cytokeratin 20 antibody [N2C2], Internal

Name: Cytokeratin 20 antibody [N2C2], Internal
Brand: GeneTex
SKU: GTX110600
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Cytokeratin 20 antibody [N2C2], Internal used in Western Blot (WB). GTX110600

Anti-Cytokeratin 20 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110600

Anti-Cytokeratin 20 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110600

Cat. No. GTX110600

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all Cytokeratin 20 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 20. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950649

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

keratin 20 , CD20 , CK-20 , CK20 , K20 , KRT21

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq]

Database

Gene ID: 54474 KRT20
UniProt: P35900 KRT20

Research Area

DATA IMAGES

GTX110600 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 20 antibody [N2C2], Internal (GTX110600) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX110600 IHC-P Image

Cytokeratin 20 antibody [N2C2], Internal detects Cytokeratin 20 protein at cytoplasm on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
Cytokeratin 20 antibody [N2C2], Internal (GTX110600) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX110600 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 20(GTX110600) antibody at 1:200 dilution.

GTX110600 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HT29 xenograft, using Cytokeratin 20(GTX110600) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Fan Y et al. Cell Death Differ 2022; Cullin 4b-RING ubiquitin ligase targets IRGM1 to regulate Wnt signaling and intestinal homeostasis.

Application : WB

Reactivity : Mouse

Yen YT et al. Cells 2020; 9 (1) : pii: E90 PP2A Deficiency Enhances Carcinogenesis of Lgr5+ Intestinal Stem Cells Both in Organoids and In Vivo.

Application : IHC-P

Reactivity : Mouse

Yen YT et al. Cells 2019; 9 (1) PP2A Deficiency Enhances Carcinogenesis of Lgr5⁺ Intestinal Stem Cells Both in Organoids and In Vivo.

Application : ICC/IF

Reactivity : Mouse

Usami S et al. J Plast Surg Hand Surg 2016; (Epub) Histological investigation of common insensate flaps obtained from the hand and forearm regions for use in fingertip reconstruction.

Rajcevic U et al. Proteome Sci 2014; 12 : 39 Colorectal cancer derived organotypic spheroids maintain essential tissue characteristics but adapt their metabolism in culture.

Application : IHC-P

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