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Cytokeratin 20 antibody [N2C2], Internal

Anti-Cytokeratin 20 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110600
Anti-Cytokeratin 20 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110600
Anti-Cytokeratin 20 antibody [N2C2], Internal used in Western Blot (WB). GTX110600
Anti-Cytokeratin 20 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110600

Cat No. GTX110600

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 20. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950649

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

keratin 20 , CD20 , CK-20 , CK20 , K20 , KRT21

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 20 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110600

GTX110600 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HT29 xenograft, using Cytokeratin 20(GTX110600) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 20 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110600

GTX110600 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 20(GTX110600) antibody at 1:200 dilution.

Anti-Cytokeratin 20 antibody [N2C2], Internal used in Western Blot (WB). GTX110600

GTX110600 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX110600 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 20 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110600

GTX110600 IHC-P Image

Cytokeratin 20 antibody [N2C2], Internal detects Cytokeratin 20 protein at cytoplasm on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
Cytokeratin 20 antibody [N2C2], Internal (GTX110600) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

Cytokeratin 20 antibody [N2C2], Internal Cat No. GTX110600

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 19-Nov-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was pear reviewed and published in the journal World J Surg Oncol in 2018.PMID: 29466986
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169