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Cytokeratin 33B antibody

Anti-Cytokeratin 33B antibody used in Western Blot (WB). GTX112454
Anti-Cytokeratin 33B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112454
Anti-Cytokeratin 33B antibody used in IHC (Paraffin sections) (IHC-P). GTX112454
Anti-Cytokeratin 33B antibody used in IHC (Paraffin sections) (IHC-P). GTX112454
Anti-Cytokeratin 33B antibody used in Western Blot (WB). GTX112454

Cat. No. GTX112454

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Dog
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 33B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 33B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166084

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 33B , HA3II , Ha-3II , K33B , KRTHA3A , KRTHA3B , hHa3-II

Background

The protein encoded by this gene is a member of the keratin gene family. It is one of the type I hair keratin genes which are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. As a type I hair keratin, it is an acidic protein which heterodimerizes with type II keratins to form hair and nails. There are two isoforms of this protein, encoded by two separate genes, KRTHA3A and KRTHA3B. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 33B antibody used in Western Blot (WB). GTX112454

GTX112454 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX112454 diluted at 1:10000

Anti-Cytokeratin 33B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112454

GTX112454 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 33B(GTX112454) antibody at 1:500 dilution.

Anti-Cytokeratin 33B antibody used in IHC (Paraffin sections) (IHC-P). GTX112454

GTX112454 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cytokeratin 33B(GTX112454) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 33B antibody used in IHC (Paraffin sections) (IHC-P). GTX112454

GTX112454 IHC-P Image

Cytokeratin 33B antibody detects Cytokeratin 33B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog squamous cell carcinoma.
Cytokeratin 33B stained by Cytokeratin 33B antibody (GTX112454) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 33B antibody used in Western Blot (WB). GTX112454

GTX112454 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 33B antibody (GTX112454) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169