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Cytokeratin 4 antibody [6B10]

Cat No. GTX11215

Host

Mouse

Clonality

Monoclonal

Clone Name

6B10

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, Dot

Reactivity

Human, Rabbit, Goat, Sheep, Cat, Dog, Hamster, Guinea pig, Pig
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:300
IHC-Fr Assay dependent
Dot Assay dependent
Not tested in other applications.

Product Note

The antibody reacts with non-cornifying squamous epithelium (e.g., exocervix, esophagus and cornea). Ciliated pseudostratified epithelium of bronchi is weakly stained. Ducts of sweat glands and prostate are focally stained. The antibody may react with some endocervical columnar cells.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

cytokeratin from human esophagus.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_374068

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

keratin 4 , CK-4 , CK4 , CYK4 , K4 , WSN1

Background

Intermediate filaments are abundant cytoplasmic structural proteins in most vertebrate cells. Cytokeratins, a group comprised of at least 29 different proteins are characteristic of epithelial and trichocytic cells. Cytokeratin 4 is a member of the type II, neutralto- basic subfamily. It is a 59 kD polypeptide differentially expressed in various human tissues. Cytokeratin 4 can be detected by biochemical or immunohistochemical means in adult non-cornified squamous epithelium such as that of the superficial and intermediate cells of the esophagus, exocervix, tongue, vagina, larynx,pharynx, epiglottis, and anus as well as in the superficial cells of the cornea. In addition, its presence was demonstrated in suprabasal cells of the urinary bladder transitional epithelium, in single cells and cell groups of sweat glands and prostatic ducts and in cylindrical ciliated bronchial cells. Cytokeratin 4 may be detected in squamous cell carcinomas derived from several non-cornified stratified epithelia. Monoclonal anti-cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal anti-Cytokeratin 4 is a chain specific antibody which may facilitate typing of normal, metaplastic and neoplastic cells.

Database

  • Gene ID: 3851 KRT4

Research Area

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 339