Cytokeratin 4 antibody

Cytokeratin 4 antibody detects KRT4 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: KRT4 protein stained by Cytokeratin 4 antibody (GTX112211) diluted at 1:500.
Blue: Hoechst 33343 staining.

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Cytokeratin 4(GTX112211) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX112211 diluted at 1:1000

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 4 antibody (GTX112211) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.