Cytokeratin 5 antibody [2C2]

Name: Cytokeratin 5 antibody [2C2]
Brand: GeneTex
SKU: GTX60580
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Cytokeratin 5 antibody [2C2] used in ELISA (ELISA). GTX60580

Anti-Cytokeratin 5 antibody [2C2] used in Flow cytometry (FACS). GTX60580

Anti-Cytokeratin 5 antibody [2C2] used in Western Blot (WB). GTX60580

Anti-Cytokeratin 5 antibody [2C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60580

Anti-Cytokeratin 5 antibody [2C2] used in IHC (Paraffin sections) (IHC-P). GTX60580

Cat. No. GTX60580

Host	Mouse
Clonality	Monoclonal
Clone Name	2C2
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human, Mouse, Monkey
Package	100 μg ($399)

See all Cytokeratin 5 products

Datasheet
Document

View product citations for antibody GTX60580 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CK5 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 5 , CK5 , DDD , DDD1 , EBS2 , K5 , KRT5A

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

Database

Gene ID: 3852 KRT5
UniProt: P13647 KRT5

Research Area

DATA IMAGES

GTX60580 ELISA Image

ELISA analysis of antigen using GTX60580 Cytokeratin 5 antibody [2C2].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

GTX60580 FACS Image

FACS analysis of HeLa cells using GTX60580 Cytokeratin 5 antibody [2C2].
Green : Cytokeratin 5
Red : negative control

GTX60580 WB Image

WB analysis of A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate using GTX60580 Cytokeratin 5 antibody [2C2].

GTX60580 WB Image

WB analysis of human CK5 (AA: 158-272) recombinant protein using GTX60580 Cytokeratin 5 antibody [2C2].

GTX60580 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60580 Cytokeratin 5 antibody [2C2].
Green : Cytokeratin 5
Red: Actin filaments

GTX60580 IHC-P Image

IHC-P analysis of breast cancer tissue using GTX60580 Cytokeratin 5 antibody [2C2].

GTX60580 IHC-P Image

IHC-P analysis of stomach cancer tissue using GTX60580 Cytokeratin 5 antibody [2C2].

REFERENCE

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REVIEW