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Cytokeratin 5 antibody [GT1486]

Anti-Cytokeratin 5 antibody [GT1486] used in Western Blot (WB). GTX634308
Anti-Cytokeratin 5 antibody [GT1486] used in IHC (Paraffin sections) (IHC-P). GTX634308
Anti-Cytokeratin 5 antibody [GT1486] used in IHC (Paraffin sections) (IHC-P). GTX634308

Cat. No. GTX634308

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1486

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

HCT-116

Predict Reactivity

Bovine, Dog, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 5. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2888435

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 5 , CK5 , DDD , DDD1 , EBS2 , K5 , KRT5A

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 5 antibody [GT1486] used in Western Blot (WB). GTX634308

GTX634308 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 5 antibody [GT1486] (GTX634308) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Cytokeratin 5 antibody [GT1486] used in IHC (Paraffin sections) (IHC-P). GTX634308

GTX634308 IHC-P Image

Cytokeratin 5 antibody [GT1486] detects Cytokeratin 5 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat thymus gland.
Cytokeratin 5 stained by Cytokeratin 5 antibody [GT1486] (GTX634308) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 5 antibody [GT1486] used in IHC (Paraffin sections) (IHC-P). GTX634308

GTX634308 IHC-P Image

Cytokeratin 5 antibody [GT1486] detects Cytokeratin 5 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
Cytokeratin 5 stained by Cytokeratin 5 antibody [GT1486] (GTX634308) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations