GeneTex
United States (US)

Cytokeratin 5 antibody [RM226]

GTX33612_20160517_IHC_18121410_707.jpg
GTX33612_20160517_WB_18121410_935.jpg
GTX33612_20160517_IHC_1_18121410_726.jpg

Cat No. GTX33612

Host Rabbit
Clonality Monoclonal
Clone Name RM226
Isotype IgG
Application WB, IHC
Reactivity Human
APPLICATION

Application Note

Immunohistochemistry (IHC): 1:100 dilution, Western Blot (WB): 1:1000 dilution.

Calculated MW

62 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts to Human Cytokeratin 5. This antibody may also react to bovine, mouse, or rat Cytokeratin, as predicted by immunogen homology.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A peptide corresponding to the C-terminus of Human Cytokeratin 5

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Keratin 5,Ck5,Ddd,Ddd1,Ebs2,K5,Krt5A,Krt5

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX33612_20160517_IHC_18121410_707.jpg

GTX33612 IHC Image

Immunohistochemical staining of FFPE human tonsil tissue sections, using Anti-Cytokeratin 5 Rabbit Monoclonal antibody RM226.

GTX33612_20160517_WB_18121410_935.jpg

GTX33612 WB Image

Western Blot of A431 cell lysates using RM226,showed a band of Cytokeratin 5 expressed in A431 cells.

GTX33612_20160517_IHC_1_18121410_726.jpg

GTX33612 IHC Image

Immunohistochemical staining of FFPE human tonsil tissue sections, using Anti-Cytokeratin 5 Rabbit Monoclonal antibody RM226.

Package List Price ($)
$ 459