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Cytokeratin 5 antibody

Anti-Cytokeratin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113219
Anti-Cytokeratin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113219
Anti-Cytokeratin 5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113219
Anti-Cytokeratin 5 antibody used in Western Blot (WB). GTX113219
Anti-Cytokeratin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113219

Cat. No. GTX113219

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Dog, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10623180

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 5 , CK5 , DDD , DDD1 , EBS2 , K5 , KRT5A

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113219

GTX113219 IHC-P Image

Cytokeratin 5 antibody detects Cytokeratin 5 protein at cytosol and membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Cytokeratin 5 antibody (GTX113219) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113219

GTX113219 IHC-P Image

Cytokeratin 5 antibody detects Cytokeratin 5 protein at cell membrane and cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Cytokeratin 5 antibody (GTX113219) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113219

GTX113219 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 5(GTX113219) antibody at 1:200 dilution.

Anti-Cytokeratin 5 antibody used in Western Blot (WB). GTX113219

GTX113219 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 5 antibody (GTX113219) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113219

GTX113219 IHC-P Image

Cytokeratin 5 antibody detects Cytokeratin 5 protein at cytosol and membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Cytokeratin 5 antibody (GTX113219) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

CH Yeh et al. Toxics 2024; 12 (4) : 239 Chlorogenic Acid Intravesical Therapy Changes Acute Voiding Behavior of Systemic Lipopolysaccharide Inflammation-Induced Cystitis Bladder in Mice
Application : IHC-P
Koterazawa Y et al. J Gastroenterol 2020; 55 (8) : 763-774 Retinoic acid receptor γ activation promotes differentiation of human induced pluripotent stem cells into esophageal epithelium.
Wang S et al. Med Sci (Paris) 2018; 34 Focus issue F1 : 94-98 Knock-down of filaggrin influences the mitogen-activated protein kinases signaling pathway in normal human epidermal keratinocytes.
Application : WB
Reactivity : Human
Meng X et al. Open Med (Wars) 2018; 13 : 189-195 MAPK Pathway Involved in Epidermal Terminal Differentiation of Normal Human Epidermal Keratinocytes.
Ordovas-Montanes J et al. bioRxiv 2017; (Epub) Reduced cellular diversity and an altered basal progenitor cell state inform epithelial barrier dysfunction in human type 2 immunity.
Application : IHC-P
Reactivity : Human
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REVIEW

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Application Reference
CH Yeh et al. Toxics 2024; 12 (4):239 Chlorogenic Acid Intravesical Therapy Changes Acute Voiding Behavior of Systemic Lipopolysaccharide Inflammation-Induced Cystitis Bladder in Mice
Application : IHC-P
Koterazawa Y et al. J Gastroenterol 2020; 55 (8):763-774 Retinoic acid receptor γ activation promotes differentiation of human induced pluripotent stem cells into esophageal epithelium.
Wang S et al. Med Sci (Paris) 2018; 34 Focus issue F1:94-98 Knock-down of filaggrin influences the mitogen-activated protein kinases signaling pathway in normal human epidermal keratinocytes.
Application : WB
Reactivity : Human
Meng X et al. Open Med (Wars) 2018; 13:189-195 MAPK Pathway Involved in Epidermal Terminal Differentiation of Normal Human Epidermal Keratinocytes.
Ordovas-Montanes J et al. bioRxiv 2017; (Epub) Reduced cellular diversity and an altered basal progenitor cell state inform epithelial barrier dysfunction in human type 2 immunity.
Application : IHC-P
Reactivity : Human
Lin YC et al. Sci Rep 2017; 7:46338 MAOA-a novel decision maker of apoptosis and autophagy in hormone refractory neuroendocrine prostate cancer cells.
Application : IHC-P
Reactivity : Human
Qian L et al. Cytokine 2017; 96:114-122 LncRNA expression profile of ΔNp63α in cervical squamous cancers and its suppressive effects on LIF expression.
Dang NN et al. Braz J Med Biol Res 2015; 48 (1):39-45 Filaggrin silencing by shRNA directly impairs the skin barrier function of normal human epidermal keratinocytes and then induces an immune response.
Dang N et al. Mol Biol Rep 2014; Knockdown of filaggrin influences the epidermal terminal differentiation via MAPK pathway in normal human epidermal keratinocytes.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169