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Cytokeratin 5 antibody

Anti-Cytokeratin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113219
Anti-Cytokeratin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113219
Anti-Cytokeratin 5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113219
Anti-Cytokeratin 5 antibody used in Western Blot (WB). GTX113219
Anti-Cytokeratin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113219

Cat No. GTX113219

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Dog, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10623180

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

keratin 5 , CK5 , DDD , DDD1 , EBS2 , K5 , KRT5A

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113219

GTX113219 IHC-P Image

Cytokeratin 5 antibody detects Cytokeratin 5 protein at cytosol and membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Cytokeratin 5 antibody (GTX113219) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113219

GTX113219 IHC-P Image

Cytokeratin 5 antibody detects Cytokeratin 5 protein at cytosol and membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Cytokeratin 5 antibody (GTX113219) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113219

GTX113219 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 5(GTX113219) antibody at 1:200 dilution.

Anti-Cytokeratin 5 antibody used in Western Blot (WB). GTX113219

GTX113219 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 5 antibody (GTX113219) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113219

GTX113219 IHC-P Image

Cytokeratin 5 antibody detects Cytokeratin 5 protein at cell membrane and cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Cytokeratin 5 antibody (GTX113219) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf