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Cytokeratin 5 antibody

Anti-Cytokeratin 5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33140
Anti-Cytokeratin 5 antibody used in Immunoprecipitation (IP). GTX33140
Anti-Cytokeratin 5 antibody used in Western Blot (WB). GTX33140
Anti-Cytokeratin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX33140

Cat. No. GTX33140

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Cytokeratin 5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein of human KRT5

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 5 , CK5 , DDD , DDD1 , EBS2 , K5 , KRT5A

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33140

GTX33140 ICC/IF Image

ICC/IF analysis of L929 cells using GTX33140 Cytokeratin 5 antibody.
Blue : DAPI
Dilution : 1:100

Anti-Cytokeratin 5 antibody used in Immunoprecipitation (IP). GTX33140

GTX33140 IP Image

IP analysis of A431 cell lysate using GTX33140 Cytokeratin 5 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:5000

Anti-Cytokeratin 5 antibody used in Western Blot (WB). GTX33140

GTX33140 WB Image

WB analysis of various sample lysates using GTX33140 Cytokeratin 5 antibody.
Dilution : 1:500
Loading : 25μg per lane

Anti-Cytokeratin 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX33140

GTX33140 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX33140 Cytokeratin 5 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399