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Cytokeratin 6 + 18 antibody

Cat. No. GTX20040

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human
Package
100 μg ($399)
See all Cytokeratin 6 + 18 products

APPLICATION

Application Note

AF: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 59 & 48 kDa respectively. Optimal dilutions/concentrations should be determined by the end user.

Positive Control

Normal skin

Product Note

Stains all human epithelial cells except for the inner hair root sheath and one or two other exceptional cell types. Does not react significantly with Keratins 1, 8 and 19.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human stratum corneum

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_423829

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

KRT6B , P04259 , P02538 , KRT18 , Cytokeratin 6 + 18 , 148041 , Cytokeratin6 + 18 , K18 , CYK18 , 3853 , K6A , PC2 , 148042 , K6B , 3854 , P05783 , CK6B , KRT6A , CK6A , 3875 , KRTL1 , 148070

Cellular Localization

Cytoplasmic

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]

Research Area

REFERENCE

Application Reference

Ordonez NG et al. Hum Pathol 2013; 44 (7) : 1195-215 Broad-spectrum immunohistochemical epithelial markers: a review.
Application : IHC-P
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Package List Price ($)
$ 399
Custom Conjugations