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Cytokeratin 6 antibody

Anti-Cytokeratin 6 antibody used in IHC (Paraffin sections) (IHC-P). GTX105355
Anti-Cytokeratin 6 antibody used in Western Blot (WB). GTX105355
Anti-Cytokeratin 6 antibody used in IHC (Paraffin sections) (IHC-P). GTX105355
Anti-Cytokeratin 6 antibody used in Western Blot (WB). GTX105355
Anti-Cytokeratin 6 antibody used in Western Blot (WB). GTX105355

Cat. No. GTX105355

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950653

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CK6A antibody , CK6C antibody , CK6D antibody , K6A antibody , K6C antibody , K6D antibody , KRT6C antibody , KRT6D antibody , KRT6A antibody , cytokeratin 6D antibody , cytokeratin 6C antibody , "keratin , epidermal type II , K6A antibody" , type-II keratin Kb6 antibody , "keratin , type II cytoskeletal 6A antibody" , cytokeratin 6A antibody , keratin 6A antibody

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-Cytokeratin 6 antibody used in IHC (Paraffin sections) (IHC-P). GTX105355

GTX105355 IHC-P Image

Cytokeratin 6 antibody detects Cytokeratin 6 protein at membrane on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
Cytokeratin 6 antibody (GTX105355) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 6 antibody used in Western Blot (WB). GTX105355

GTX105355 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX105355 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 6 antibody used in IHC (Paraffin sections) (IHC-P). GTX105355

GTX105355 IHC-P Image

Immunohistochemical analysis of paraffin-embedded D54MG xenograft, using KRT6A(GTX105355) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 6 antibody used in Western Blot (WB). GTX105355

GTX105355 WB Image

The data was published in the journal PLoS One in 2015. PMID: 26046540

Anti-Cytokeratin 6 antibody used in Western Blot (WB). GTX105355

GTX105355 WB Image

The data was published in the journal PLoS One in 2015. PMID: 26046540

REFERENCE

Application Reference

Seo J et al. Wound Repair Regen 2016; (Epub) Examination of endothelial cell induced epidermal regeneration in a mice based chimney wound model.
Application : IHC-P
Reactivity : Mouse
Chen Y et al. PLoS One 2015; 10 (6) : e0127957 Downregulation of TNIP1 Expression Leads to Increased Proliferation of Human Keratinocytes and Severer Psoriasis-Like Conditions in an Imiquimod-Induced Mouse Model of Dermatitis.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169