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Cytokeratin 6 antibody

Anti-Cytokeratin 6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110426
Anti-Cytokeratin 6 antibody used in Western Blot (WB). GTX110426
Anti-Cytokeratin 6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110426

Cat. No. GTX110426

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 6B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167398

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CK6B antibody , K6B antibody , KRTL1 antibody , PC2 antibody , KRT6B antibody , "keratin , epidermal , type II , K6B antibody" , keratin-6B antibody , cytokeratin-6B antibody , cytokeratin 6B antibody , type-II keratin Kb10 antibody , "keratin , type II cytoskeletal 6B antibody" , keratin-like 1 (a type II keratin sequence) antibody , keratin 6B antibody

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-Cytokeratin 6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110426

GTX110426 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse brain, using Cytokeratin 6 (GTX110426) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 6 antibody used in Western Blot (WB). GTX110426

GTX110426 WB Image

Sample (1 μg of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX110426 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110426

GTX110426 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 6 (GTX110426) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169