APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Cytokeratin 6B. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11167398
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CK6B antibody , K6B antibody , KRTL1 antibody , PC2 antibody , KRT6B antibody , "keratin , epidermal , type II , K6B antibody" , keratin-6B antibody , cytokeratin-6B antibody , cytokeratin 6B antibody , type-II keratin Kb10 antibody , "keratin , type II cytoskeletal 6B antibody" , keratin-like 1 (a type II keratin sequence) antibody , keratin 6B antibody
Background
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]
Research Area
DATA IMAGES
|
GTX110426 IHC-P Image
Immunohistochemical analysis of paraffin-embedded mouse brain, using Cytokeratin 6 (GTX110426) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
|
GTX110426 WB Image
Sample (1 μg of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX110426 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX110426 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 6 (GTX110426) antibody at 1:500 dilution.
|
REFERENCE
There are currently no references for Cytokeratin 6 antibody (GTX110426). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cytokeratin 6 antibody (GTX110426). Be the first to share your experience with this product.
