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Cytokeratin 7 antibody [5D12]

Anti-Cytokeratin 7 antibody [5D12] used in Western Blot (WB). GTX83354
Anti-Cytokeratin 7 antibody [5D12] used in Flow cytometry (FACS). GTX83354

Cat. No. GTX83354

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5D12

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Cytokeratin 7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CK7 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 7 , CK7 , K2C7 , K7 , SCL

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 7 antibody [5D12] used in Western Blot (WB). GTX83354

GTX83354 WB Image

WB analysis of HeLa (1), MCF-7 (2), A431 (3) and A549 (4) cell lysate using GTX83354 Cytokeratin 7 antibody [5D12].

Anti-Cytokeratin 7 antibody [5D12] used in Flow cytometry (FACS). GTX83354

GTX83354 FACS Image

FACS analysis of HeLa cells using GTX83354 Cytokeratin 7 antibody [5D12].
Green : Cytokeratin 7
Purple : negative control

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399