GeneTex

  • Country / Location Selection

United States (US)

Cytokeratin 7 antibody [KRT7/1198]

Anti-Cytokeratin 7 antibody [KRT7/1198] used in Western Blot (WB). GTX34660
Anti-Cytokeratin 7 antibody [KRT7/1198] used in IHC (Paraffin sections) (IHC-P). GTX34660
Anti-Cytokeratin 7 antibody [KRT7/1198] used in IHC (Paraffin sections) (IHC-P). GTX34660
Anti-Cytokeratin 7 antibody [KRT7/1198] used in IHC (Paraffin sections) (IHC-P). GTX34660
Anti-Cytokeratin 7 antibody [KRT7/1198] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34660

Cat. No. GTX34660

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 KRT7/1198

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Rat
Package
100 μg ($529)
See all Cytokeratin 7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Product Note

This antibody recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human KRT7 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

RRID

AB_2887741

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CK7 , K2C7 , K7 , KRT7 , SCL , keratin 7 , Cytokeratin 7

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 7 antibody [KRT7/1198] used in Western Blot (WB). GTX34660

GTX34660 WB Image

WB analysis of HeLa cell lysate using GTX34660 Cytokeratin 7 antibody [KRT7/1198].

Anti-Cytokeratin 7 antibody [KRT7/1198] used in IHC (Paraffin sections) (IHC-P). GTX34660

GTX34660 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX34660 Cytokeratin 7 antibody [KRT7/1198].

Anti-Cytokeratin 7 antibody [KRT7/1198] used in IHC (Paraffin sections) (IHC-P). GTX34660

GTX34660 IHC-P Image

IHC-P analysis of rat lung tissue using GTX34660 Cytokeratin 7 antibody [KRT7/1198].

Anti-Cytokeratin 7 antibody [KRT7/1198] used in IHC (Paraffin sections) (IHC-P). GTX34660

GTX34660 IHC-P Image

IHC-P analysis of human ovarian carcinoma tissue using GTX34660 Cytokeratin 7 antibody [KRT7/1198].

Anti-Cytokeratin 7 antibody [KRT7/1198] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34660

GTX34660 ICC/IF Image

ICC/IF analysis of MeOH-fixed HeLa cells using GTX34660 Cytokeratin 7 antibody [KRT7/1198].
Green : Primary antibody
Red : nucleus

REFERENCE

Application Reference

Hu Z et al. Mol Ther Nucleic Acids 2019; 17 : 198-209 ssODN-Mediated In-Frame Deletion with CRISPR/Cas9 Restores FVIII Function in Hemophilia A-Patient-Derived iPSCs and ECs.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Cytokeratin 7 antibody [KRT7/1198] (GTX34660). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529