Cytokeratin 7 antibody [KRT7/1499R]

Cat. No. GTX34661

Host	Rabbit
Clonality	Monoclonal
Clone Name	KRT7/1499R
Isotype	IgG
Application	WB, IHC-P, FACS
Reactivity	Human
Package	100 μg ($529)

See all Cytokeratin 7 products

Datasheet
Document

View product citations for antibody GTX34661 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
IHC-P	1-2μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
Note : IHC-P Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Product Note

This antibody recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human KRT7 protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CK7 , K2C7 , K7 , KRT7 , SCL , keratin 7 , Cytokeratin 7

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

Database

Gene ID: 3855 KRT7
UniProt: P08729 KRT7

Research Area

DATA IMAGES

GTX34661 WB Image

WB analysis of HeLa cell lysate using GTX34661 Cytokeratin 7 antibody [KRT7/1499R].

GTX34661 FACS Image

FACS analysis of PFA-fixed HeLa cells using GTX34661 Cytokeratin 7 antibody [KRT7/1499R].
Blue : Primary antibody
Red : Isotype control

GTX34661 IHC-P Image

IHC-P analysis of human oviduct tissue using GTX34661 Cytokeratin 7 antibody [KRT7/1499R].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty