GeneTex
United States (US)

Cytokeratin 7 antibody [KRT7/1499R]

GTX34661_20170712_IHC-P_18121410_452.jpg

Cat No. GTX34661

Host Rabbit
Clonality Monoclonal
Clone Name KRT7/1499R
Isotype IgG
Application IHC-P
Reactivity Human
APPLICATION

Application Note

IHC-P:0.5-1.0ug/ml for 30 minutes at RT.

Calculated MW

51 kDa. ( Note )

Specificity/Sensitivity

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full-length human KRT7 protein

Purification

Ab purified by Protein A Column

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Keratin 7,Ck7,K2C7,K7,Scl,Krt7

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX34661_20170712_IHC-P_18121410_452.jpg

GTX34661 IHC-P Image

Formalin-fixed, paraffin-embedded human Oviduct stained with Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody (KRT7/1499R)

Package List Price ($)
$ 379