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Cytokeratin 7 antibody [KRT7/760]

Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659
Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659
Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659

Cat No. GTX34659

Host Mouse
Clonality Monoclonal
Clone Name KRT7/760
Isotype IgG1
Application ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Specificity/Sensitivity

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full-length human KRT7 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Keratin 7,Ck7,K2C7,K7,Scl,Krt7

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659

GTX34659 IHC-P Image

Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/760)

Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659

GTX34659 IHC-P Image

Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/760)

Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659

GTX34659 IHC-P Image

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/760)

Package List Price ($)
$ 329