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Cytokeratin 7 antibody [KRT7/760]

Anti-Cytokeratin 7 antibody [KRT7/760] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34659
Anti-Cytokeratin 7 antibody [KRT7/760] used in Flow cytometry (FACS). GTX34659
Anti-Cytokeratin 7 antibody [KRT7/760] used in Western Blot (WB). GTX34659
Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659
Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659
Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659

Cat No. GTX34659

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 KRT7/760

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($339)
See all Cytokeratin 7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human KRT7 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CK7 , K2C7 , K7 , KRT7 , SCL , keratin 7 , Cytokeratin 7

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 7 antibody [KRT7/760] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34659

GTX34659 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX34659 Cytokeratin 7 antibody [KRT7/760].
Green : Primary antibody
Red : nucleus

Anti-Cytokeratin 7 antibody [KRT7/760] used in Flow cytometry (FACS). GTX34659

GTX34659 FACS Image

FACS analysis of paraformaldehyde-fixed HeLa cells using GTX34659 Cytokeratin 7 antibody [KRT7/760].
Blue : Primary antibody
Red : Isotype control

Anti-Cytokeratin 7 antibody [KRT7/760] used in Western Blot (WB). GTX34659

GTX34659 WB Image

WB analysis of HeLa cell lysate using GTX34659 Cytokeratin 7 antibody [KRT7/760].

Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659

GTX34659 IHC-P Image

IHC-P analysis of human endometrial carcinoma tissue using GTX34659 Cytokeratin 7 antibody [KRT7/760].

Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659

GTX34659 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX34659 Cytokeratin 7 antibody [KRT7/760].

Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659

GTX34659 IHC-P Image

IHC-P analysis of human ovarian carcinoma tissue using GTX34659 Cytokeratin 7 antibody [KRT7/760].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339