APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
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Not tested in other applications.
Calculated MW
Product Note
This antibody recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human KRT7 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CK7 , K2C7 , K7 , KRT7 , SCL , keratin 7 , Cytokeratin 7
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Cytokeratin 7 antibody [KRT7/760] used in Western Blot (WB). GTX34659](/templates/default/js/lightbox/images/loading.gif "Anti-Cytokeratin 7 antibody [KRT7/760] used in Western Blot (WB). GTX34659")
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GTX34659 WB Image
WB analysis of HeLa cell lysate using GTX34659 Cytokeratin 7 antibody [KRT7/760].
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GTX34659 IHC-P Image
IHC-P analysis of human endometrial carcinoma tissue using GTX34659 Cytokeratin 7 antibody [KRT7/760].
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GTX34659 IHC-P Image
IHC-P analysis of human lung carcinoma tissue using GTX34659 Cytokeratin 7 antibody [KRT7/760].
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GTX34659 IHC-P Image
IHC-P analysis of human ovarian carcinoma tissue using GTX34659 Cytokeratin 7 antibody [KRT7/760].
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GTX34659 ICC/IF Image
ICC/IF analysis of methanol-fixed HeLa cells using GTX34659 Cytokeratin 7 antibody [KRT7/760].
Green : Primary antibody
Red : nucleus
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GTX34659 FACS Image
FACS analysis of paraformaldehyde-fixed HeLa cells using GTX34659 Cytokeratin 7 antibody [KRT7/760].
Blue : Primary antibody
Red : Isotype control
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REFERENCE
There are currently no references for Cytokeratin 7 antibody [KRT7/760] (GTX34659). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cytokeratin 7 antibody [KRT7/760] (GTX34659). Be the first to share your experience with this product.
![Anti-Cytokeratin 7 antibody [KRT7/760] used in Western Blot (WB). GTX34659](/upload/website/prouct_img/small/GTX34659/GTX34659_20200115_WB_141_w_23051500_306.webp "Anti-Cytokeratin 7 antibody [KRT7/760] used in Western Blot (WB). GTX34659")
![Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659](/upload/website/prouct_img/small/GTX34659/GTX34659_20200115_IHC-P_537_w_23051500_261.webp "Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659")
![Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659](/upload/website/prouct_img/small/GTX34659/GTX34659_20200115_IHC-P_680_w_23051500_589.webp "Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659")
![Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659](/upload/website/prouct_img/small/GTX34659/GTX34659_20200115_IHC-P_809_w_23051500_600.webp "Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659")
![Anti-Cytokeratin 7 antibody [KRT7/760] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34659](/upload/website/prouct_img/small/GTX34659/GTX34659_20200115_ICC-IF_1602_w_23051500_740.webp "Anti-Cytokeratin 7 antibody [KRT7/760] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34659")
![Anti-Cytokeratin 7 antibody [KRT7/760] used in Flow cytometry (FACS). GTX34659](/upload/website/prouct_img/small/GTX34659/GTX34659_20200115_FACS_1654_w_23051500_328.webp "Anti-Cytokeratin 7 antibody [KRT7/760] used in Flow cytometry (FACS). GTX34659")
![Anti-Cytokeratin 7 antibody [KRT7/760] used in Western Blot (WB). GTX34659](/upload/website/prouct_img/normal/GTX34659/GTX34659_20200115_WB_141_w_23051500_306.webp "Anti-Cytokeratin 7 antibody [KRT7/760] used in Western Blot (WB). GTX34659")
![Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659](/upload/website/prouct_img/normal/GTX34659/GTX34659_20200115_IHC-P_537_w_23051500_261.webp "Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659")
![Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659](/upload/website/prouct_img/normal/GTX34659/GTX34659_20200115_IHC-P_680_w_23051500_589.webp "Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659")
![Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659](/upload/website/prouct_img/normal/GTX34659/GTX34659_20200115_IHC-P_809_w_23051500_600.webp "Anti-Cytokeratin 7 antibody [KRT7/760] used in IHC (Paraffin sections) (IHC-P). GTX34659")
![Anti-Cytokeratin 7 antibody [KRT7/760] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34659](/upload/website/prouct_img/normal/GTX34659/GTX34659_20200115_ICC-IF_1602_w_23051500_740.webp "Anti-Cytokeratin 7 antibody [KRT7/760] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34659")
![Anti-Cytokeratin 7 antibody [KRT7/760] used in Flow cytometry (FACS). GTX34659](/upload/website/prouct_img/normal/GTX34659/GTX34659_20200115_FACS_1654_w_23051500_328.webp "Anti-Cytokeratin 7 antibody [KRT7/760] used in Flow cytometry (FACS). GTX34659")