Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 9.0 showed a strongest staining pattern. However, pH 9.0 results in only a slight reduction of the staining intensity as compared to pH 9.0. Therefore, the pH 7.8 was an optimal for manual staining because of the better tissue preservation at pH 7.8 than at pH 9.0.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Pancreas: CK7 staining is at least weak to moderate cytoplasmic intercalating ducts and strong in large pancreatic ducts.
Negative Control
Pancreas: CK7 staining should be absent in all acinar cells.
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa1-100) of human KRT7 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
keratin 7 , CK7 , K2C7 , K7 , SCL
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
Database
Research Area
![Anti-Cytokeratin 7 antibody [MSVA-607R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04375](/upload/website/prouct_img/small/GTX04375/GTX04375_20230728_IHC-P_294_23072723_882.webp "Anti-Cytokeratin 7 antibody [MSVA-607R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04375")
![Anti-Cytokeratin 7 antibody [MSVA-607R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04375](/upload/website/prouct_img/small/GTX04375/GTX04375_20230728_IHC-P_34_23072722_645.webp "Anti-Cytokeratin 7 antibody [MSVA-607R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04375")
![Anti-Cytokeratin 7 antibody [MSVA-607R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04375](/upload/website/prouct_img/small/GTX04375/GTX04375_20230728_IHC-P_164_23072722_458.webp "Anti-Cytokeratin 7 antibody [MSVA-607R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04375")
![Anti-Cytokeratin 7 antibody [MSVA-607R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04375](/upload/website/prouct_img/normal/GTX04375/GTX04375_20230728_IHC-P_294_23072723_882.webp "Anti-Cytokeratin 7 antibody [MSVA-607R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04375")
![Anti-Cytokeratin 7 antibody [MSVA-607R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04375](/upload/website/prouct_img/normal/GTX04375/GTX04375_20230728_IHC-P_34_23072722_645.webp "Anti-Cytokeratin 7 antibody [MSVA-607R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04375")
![Anti-Cytokeratin 7 antibody [MSVA-607R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04375](/upload/website/prouct_img/normal/GTX04375/GTX04375_20230728_IHC-P_164_23072722_458.webp "Anti-Cytokeratin 7 antibody [MSVA-607R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04375")