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Cytokeratin 7 antibody [N1C2]

Anti-Cytokeratin 7 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX109723
Anti-Cytokeratin 7 antibody [N1C2] used in Western Blot (WB). GTX109723
Anti-Cytokeratin 7 antibody [N1C2] used in IHC (Frozen sections) (IHC-Fr). GTX109723
Anti-Cytokeratin 7 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109723
Anti-Cytokeratin 7 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX109723

Cat No. GTX109723

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

A431 , HeLa , Molt-4

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950654

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

keratin 7 , CK7 , K2C7 , K7 , SCL

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 7 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX109723

GTX109723 IHC-P Image

Immunohistochemical characterization of Synaptophysin (GTX100865), p63 (GTX102425) and Cytokeratin 7 (GTX109723) in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens.
Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel).
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody at 1:500 overnight at 4ºC and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 7 antibody [N1C2] used in Western Blot (WB). GTX109723

GTX109723 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N1C2] (GTX109723) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 7 antibody [N1C2] used in IHC (Frozen sections) (IHC-Fr). GTX109723

GTX109723 IHC-Fr Image

Immunohistochemical analysis of human placenta (paraformaldehyde-fixed frozen sections), using Cytokeratin 7(GTX109723) antibody at 1:200 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 7 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109723

GTX109723 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 7(GTX109723) antibody at 1:200 dilution.

Anti-Cytokeratin 7 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX109723

GTX109723 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Cytokeratin 7(GTX109723) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169