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Cytokeratin 7 antibody [N2C2], Internal

KO/KD
Anti-Cytokeratin 7 antibody [N2C2], Internal used in Western Blot (WB). GTX110414
Anti-Cytokeratin 7 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110414
Anti-Cytokeratin 7 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110414
Anti-Cytokeratin 7 antibody [N2C2], Internal used in Western Blot (WB). GTX110414

Cat. No. GTX110414

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Bovine, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164456

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 7 , CK7 , K2C7 , K7 , SCL

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 7 antibody [N2C2], Internal used in Western Blot (WB). GTX110414

GTX110414 WB Image

KO/KD

Wild-type (WT) and Cytokeratin 7 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N2C2], Internal (GTX110414) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 7 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110414

GTX110414 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 7(GTX110414) antibody at 1:500 dilution.

Anti-Cytokeratin 7 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110414

GTX110414 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using Cytokeratin 7(GTX110414) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 7 antibody [N2C2], Internal used in Western Blot (WB). GTX110414

GTX110414 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N2C2], Internal (GTX110414) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Casique-Aguirre D et al. BMC Cancer 2018; 18 (1) : 1299 KRas4B-PDE6δ complex stabilization by small molecules obtained by virtual screening affects Ras signaling in pancreatic cancer.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169