Wild-type (WT) and Cytokeratin 7 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N2C2], Internal (GTX110414) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N2C2], Internal (GTX110414) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using Cytokeratin 7(GTX110414) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 7(GTX110414) antibody at 1:500 dilution.