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Cytokeratin 8 antibody [1B9]

KO/KD Overexpression
Anti-Cytokeratin 8 antibody [1B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84247
Anti-Cytokeratin 8 antibody [1B9] used in Western Blot (WB). GTX84247
Anti-Cytokeratin 8 antibody [1B9] used in Western Blot (WB). GTX84247
Anti-Cytokeratin 8 antibody [1B9] used in Western Blot (WB). GTX84247

Cat. No. GTX84247

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1B9

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human, Dog, Monkey
Package
100 μl ($519)
See all Cytokeratin 8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2500-1:5000
ICC/IF 1:100
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 8 , CARD2 , CK-8 , CK8 , CYK8 , K2C8 , K8 , KO

Cellular Localization

Cytoplasm,nucleoplasm

Background

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 8 antibody [1B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84247

GTX84247 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Cytokeratin 8 plasmid using GTX84247 Cytokeratin 8 antibody [1B9].

Anti-Cytokeratin 8 antibody [1B9] used in Western Blot (WB). GTX84247

GTX84247 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Cytokeratin 8 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84247 Cytokeratin 8 antibody [1B9].
Loading : 5 ug per lane

Anti-Cytokeratin 8 antibody [1B9] used in Western Blot (WB). GTX84247

GTX84247 WB Image

KO/KD

WB analysis of wild type and Cytokeratin 8 knock out HeLa cell lysate(10μg per lane) using GTX84247 Cytokeratin 8 antibody [1B9].
Dilution : 1:2000

Anti-Cytokeratin 8 antibody [1B9] used in Western Blot (WB). GTX84247

GTX84247 WB Image

WB analysis of various cell lines using GTX84247 Cytokeratin 8 antibody [1B9].
Loading : 35 ug per lane
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519