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Cytokeratin 8 antibody [4D12]

KO/KD Overexpression
Anti-Cytokeratin 8 antibody [4D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84248
Anti-Cytokeratin 8 antibody [4D12] used in IHC (Paraffin sections) (IHC-P). GTX84248
Anti-Cytokeratin 8 antibody [4D12] used in Western Blot (WB). GTX84248
Anti-Cytokeratin 8 antibody [4D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84248
Anti-Cytokeratin 8 antibody [4D12] used in Western Blot (WB). GTX84248
Anti-Cytokeratin 8 antibody [4D12] used in Western Blot (WB). GTX84248

Cat. No. GTX84248

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4D12

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Dog, Monkey
Package
100 μl ($519)
See all Cytokeratin 8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

keratin 8 , CARD2 , CK-8 , CK8 , CYK8 , K2C8 , K8 , KO

Cellular Localization

Cytoplasm,nucleoplasm

Background

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 8 antibody [4D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84248

GTX84248 ICC/IF Image

ICC/IF analysis of A549 cells using GTX84248 Cytokeratin 8 antibody [4D12].

Anti-Cytokeratin 8 antibody [4D12] used in IHC (Paraffin sections) (IHC-P). GTX84248

GTX84248 IHC-P Image

IHC-P analysis of human ovary tissue using GTX84248 Cytokeratin 8 antibody [4D12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 8 antibody [4D12] used in Western Blot (WB). GTX84248

GTX84248 WB Image

WB analysis of various cell lines using GTX84248 Cytokeratin 8 antibody [4D12].
Loading : 35 ug per lane

Anti-Cytokeratin 8 antibody [4D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84248

GTX84248 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Cytokeratin 8 plasmid using GTX84248 Cytokeratin 8 antibody [4D12].

Anti-Cytokeratin 8 antibody [4D12] used in Western Blot (WB). GTX84248

GTX84248 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Cytokeratin 8 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84248 Cytokeratin 8 antibody [4D12].
Loading : 5 ug per lane

Anti-Cytokeratin 8 antibody [4D12] used in Western Blot (WB). GTX84248

GTX84248 WB Image

KO/KD

WB analysis of wild type and Cytokeratin 8 knock out HeLa cell lysate(10μg per lane) using GTX84248 Cytokeratin 8 antibody [4D12].
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519