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Cytokeratin 8 antibody [8A5D12]

Anti-Cytokeratin 8 antibody [8A5D12] used in IHC (Paraffin sections) (IHC-P). GTX83140
Anti-Cytokeratin 8 antibody [8A5D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83140
Anti-Cytokeratin 8 antibody [8A5D12] used in IHC (Paraffin sections) (IHC-P). GTX83140
Anti-Cytokeratin 8 antibody [8A5D12] used in Western Blot (WB). GTX83140

Cat. No. GTX83140

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8A5D12

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($409)
See all Cytokeratin 8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human Cytokeratin (aa391-483) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 8 , CARD2 , CK-8 , CK8 , CYK8 , K2C8 , K8 , KO

Cellular Localization

Cytoplasm,nucleoplasm

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 8 antibody [8A5D12] used in IHC (Paraffin sections) (IHC-P). GTX83140

GTX83140 IHC-P Image

IHC-P analysis of human Kidney tissue using GTX83140 Cytokeratin 8 antibody [8A5D12].

Anti-Cytokeratin 8 antibody [8A5D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83140

GTX83140 ICC/IF Image

ICC/IF analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using GTX83140 Cytokeratin 8 antibody [8A5D12].
Green : Cytokeratin 8
Blue: DRAQ5 fluorescent DNA dye

Anti-Cytokeratin 8 antibody [8A5D12] used in IHC (Paraffin sections) (IHC-P). GTX83140

GTX83140 IHC-P Image

IHC-P analysis of human breast carcinoma (A), lung cancer (B) and ovarian cancer tissue (C) using GTX83140 Cytokeratin 8 antibody [8A5D12].

Anti-Cytokeratin 8 antibody [8A5D12] used in Western Blot (WB). GTX83140

GTX83140 WB Image

WB analysis of A549 (1), HeLa (2), MCF-7 (3), A431 (4) and HepG2 (5) cell lysate using GTX83140 Cytokeratin 8 antibody [8A5D12].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409