APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Recommended starting dilution is 1:1000. |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT3D11 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KRT10 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
keratin 8 , CARD2 , CK8 , CYK8 , K2C8 , K8 , KO , KRT8 , Cytokeratin 8 , Keratin 8
Cellular Localization
Cytoplasm,Nucleus,Nucleoplasm,Nucleus matrix
Background
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
Database
Research Area
DATA IMAGES
![Anti-Cytokeratin 8 antibody [AT3D11] used in Flow cytometry (FACS). GTX57589](/templates/default/js/lightbox/images/loading.gif "Anti-Cytokeratin 8 antibody [AT3D11] used in Flow cytometry (FACS). GTX57589")
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GTX57589 FACS Image
FACS analysis of MCF7 cells using GTX57589 Cytokeratin 8 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg
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GTX57589 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57589 Cytokeratin 8 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57589 WB Image
WB analysis of HeLa whole cell lysate using GTX57589 Cytokeratin 8 antibody.
Loading : 40 μg
Dilution : 1:1000
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GTX57589 WB Image
WB analysis of various samples using GTX57589 Cytokeratin 8 antibody.
Lane 1 : LNCap whole cell lysate
Lane 2 : PC-3 whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : A549 whole cell lysate
Loading : 40 μg
Dilution : 1:1000
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GTX57589 ICC/IF Image
ICC/IF analysis of MCF7 cells using GTX57589 Cytokeratin 8 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
|
REFERENCE
There are currently no references for Cytokeratin 8 antibody [AT3D11] (GTX57589). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cytokeratin 8 antibody [AT3D11] (GTX57589). Be the first to share your experience with this product.
![Anti-Cytokeratin 8 antibody [AT3D11] used in Flow cytometry (FACS). GTX57589](/upload/website/prouct_img/small/GTX57589/GTX57589_20200204_FC_w_23061123_911.webp "Anti-Cytokeratin 8 antibody [AT3D11] used in Flow cytometry (FACS). GTX57589")
![Anti-Cytokeratin 8 antibody [AT3D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57589](/upload/website/prouct_img/small/GTX57589/GTX57589_20200204_IF2_w_23061123_912.webp "Anti-Cytokeratin 8 antibody [AT3D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57589")
![Anti-Cytokeratin 8 antibody [AT3D11] used in Western Blot (WB). GTX57589](/upload/website/prouct_img/small/GTX57589/GTX57589_20200204_WB1_w_23061123_338.webp "Anti-Cytokeratin 8 antibody [AT3D11] used in Western Blot (WB). GTX57589")
![Anti-Cytokeratin 8 antibody [AT3D11] used in Western Blot (WB). GTX57589](/upload/website/prouct_img/small/GTX57589/GTX57589_20200204_WB2_w_23061123_576.webp "Anti-Cytokeratin 8 antibody [AT3D11] used in Western Blot (WB). GTX57589")
![Anti-Cytokeratin 8 antibody [AT3D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57589](/upload/website/prouct_img/small/GTX57589/GTX57589_20200204_IF1_w_23061123_557.webp "Anti-Cytokeratin 8 antibody [AT3D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57589")
![Anti-Cytokeratin 8 antibody [AT3D11] used in Flow cytometry (FACS). GTX57589](/upload/website/prouct_img/normal/GTX57589/GTX57589_20200204_FC_w_23061123_911.webp "Anti-Cytokeratin 8 antibody [AT3D11] used in Flow cytometry (FACS). GTX57589")
![Anti-Cytokeratin 8 antibody [AT3D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57589](/upload/website/prouct_img/normal/GTX57589/GTX57589_20200204_IF2_w_23061123_912.webp "Anti-Cytokeratin 8 antibody [AT3D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57589")
![Anti-Cytokeratin 8 antibody [AT3D11] used in Western Blot (WB). GTX57589](/upload/website/prouct_img/normal/GTX57589/GTX57589_20200204_WB1_w_23061123_338.webp "Anti-Cytokeratin 8 antibody [AT3D11] used in Western Blot (WB). GTX57589")
![Anti-Cytokeratin 8 antibody [AT3D11] used in Western Blot (WB). GTX57589](/upload/website/prouct_img/normal/GTX57589/GTX57589_20200204_WB2_w_23061123_576.webp "Anti-Cytokeratin 8 antibody [AT3D11] used in Western Blot (WB). GTX57589")
![Anti-Cytokeratin 8 antibody [AT3D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57589](/upload/website/prouct_img/normal/GTX57589/GTX57589_20200204_IF1_w_23061123_557.webp "Anti-Cytokeratin 8 antibody [AT3D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57589")