Cytokeratin 8 antibody [K8/383]

Cat No. GTX34666

Host	Mouse
Clonality	Monoclonal
Clone Name	K8/383
Isotype	IgG1
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μg ($429)

See all Cytokeratin 8 products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ICC/IF	1-2ug/ml
IHC-P	1-2ug/ml for 30 minutes at RT
Note : IHC-P Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Specificity/Sensitivity

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (�ring-like, perinuclear� ) from ductal (�peripheral-predominant�) carcinoma of the breast.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant human full-length cytokeratin 8 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Keratin 8 , Card2 , Ck-8 , Ck8 , Cyk8 , K2C8 , K8 , Ko , Krt8

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Gene ID: 3856
UniProt: P05787

Research Area

DATA IMAGES

GTX34666 IHC-P Image

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 8 Monoclonal Antibody (K8/383).

GTX34666 WB Image

Western Blot Analysis of A431 Cell Lysate using Cytokeratin 8 Monoclonal Antibody (K8/383).

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REVIEW

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Package

List Price ($)

100 μg

$ 429

Qty

Cytokeratin 8 antibody [K8/383]

Cat No. GTX34666

Host

Clonality

Clone Name

Isotype

Application

Reactivity