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Cytokeratin 8 antibody [KRT8/4067R]

Anti-Cytokeratin 8 antibody [KRT8/4067R] used in IHC (Paraffin sections) (IHC-P). GTX02668
Anti-Cytokeratin 8 antibody [KRT8/4067R] used in IHC (Paraffin sections) (IHC-P). GTX02668
Anti-Cytokeratin 8 antibody [KRT8/4067R] used in Western Blot (WB). GTX02668

Cat. No. GTX02668

Host

Rabbit

Clonality

Monoclonal

Clone Name

KRT8/4067R

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μg ($529)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2-4 μg/ml
ICC/IF 1-2 μg/ml
IHC-P 1-2 μg/ml
FACS 1-2 μg/million cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Product Note

We do not recommend use of this product for Rat samples.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Keratin preparation from a human carcinoma. An epitope of this antibody is located between aa343-357.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 8 , CARD2 , CK-8 , CK8 , CYK8 , K2C8 , K8 , KO

Cellular Localization

Cytoplasm , nucleoplasm

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 8 antibody [KRT8/4067R] used in IHC (Paraffin sections) (IHC-P). GTX02668

GTX02668 IHC-P Image

IHC-P analysis of human colon tissue section using GTX02668 Cytokeratin 8 antibody [KRT8/4067R].

Anti-Cytokeratin 8 antibody [KRT8/4067R] used in IHC (Paraffin sections) (IHC-P). GTX02668

GTX02668 IHC-P Image

IHC-P analysis of human colon tissue section using GTX02668 Cytokeratin 8 antibody [KRT8/4067R].

Anti-Cytokeratin 8 antibody [KRT8/4067R] used in Western Blot (WB). GTX02668

GTX02668 WB Image

WB analysis of HCT116 whole cell lysate using GTX02668 Cytokeratin 8 antibody [KRT8/4067R].

REFERENCE

REVIEW

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Package List Price ($)
$ 529