GeneTex
United States (US)

Cytokeratin 8 antibody [KRT8/803]

GTX34671_20170712_IHC-P_18121410_612.jpg

Cat No. GTX34671

Host Mouse
Clonality Monoclonal
Clone Name KRT8/803
Isotype IgG1
Application IHC-P
Reactivity Human
APPLICATION

Application Note

IHC-P:0.5-1.0ug/ml for 30 min at RT.

Calculated MW

54 kDa. ( Note )

Specificity/Sensitivity

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Anti-CK8 is useful for the differentiation of lobular (�ring-like, perinuclear�) from ductal (�peripheral-predominant�) carcinoma of the breast.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human KRT8 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Keratin 8,Card2,Ck-8,Ck8,Cyk8,K2C8,K8,Ko,Krt8

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES
GTX34671_20170712_IHC-P_18121410_612.jpg

GTX34671 IHC-P Image

Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Cytokeratin 8 Monoclonal Antibody (KRT8/803).

Package List Price ($)
$ 329