GeneTex
United States (US)

Cytokeratin 8 antibody [N1C1]

KO/KD
GTX112975_40093_20170309_WB_KO_watermark_18121410_834.jpg
GTX112975_40093_WB_M_liver_18121410_455.jpg
GTX112975_40093_20151231_WB_R_liver_18121410_786.jpg
GTX112975_IFA_18121410_977.jpg
GTX112975_40093_IHC_18121410_511.jpg
GTX112975_43271_20180706_WB_18121410_227.jpg

Cat No. GTX112975

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HepG2 , HeLa , mouse liver , rat liver

Predict Reactivity

Zebrafish, Bovine, Dog, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.13mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CARD2 antibody, CK8 antibody, CYK8 antibody, K2C8 antibody, K8 antibody, KO antibody, KRT8 antibody, cytokeratin-8 antibody, type-II keratin Kb8 antibody, cytokeratin 8 antibody, "keratin, type II cytoskeletal 8 antibody", keratin 8 antibody

Cellular Localization

Cytoplasm

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX112975_40093_20170309_WB_KO_watermark_18121410_834.jpg

GTX112975 WB Image

KO/KD

Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] (GTX112975) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX112975_40093_WB_M_liver_18121410_455.jpg

GTX112975 WB Image

Sample (50 µg of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX112975 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112975_40093_20151231_WB_R_liver_18121410_786.jpg

GTX112975 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] (GTX112975) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112975_IFA_18121410_977.jpg

GTX112975 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 8(GTX112975) antibody at 1:200 dilution.

GTX112975_40093_IHC_18121410_511.jpg

GTX112975 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Cytokeratin 8(GTX112975) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112975_43271_20180706_WB_18121410_227.jpg

GTX112975 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] (GTX112975) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169