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Cytokeratin 8 antibody [N1C1]

KO/KD
Anti-Cytokeratin 8 antibody [N1C1] used in Western Blot (WB). GTX112975
Anti-Cytokeratin 8 antibody [N1C1] used in Western Blot (WB). GTX112975
Anti-Cytokeratin 8 antibody [N1C1] used in Western Blot (WB). GTX112975
Anti-Cytokeratin 8 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112975
Anti-Cytokeratin 8 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112975
Anti-Cytokeratin 8 antibody [N1C1] used in Western Blot (WB). GTX112975
Anti-Cytokeratin 8 antibody [N1C1] used in Western Blot (WB). GTX112975

Cat. No. GTX112975

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Mouse liver , rat liver

Predict Reactivity

Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950655

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 8 , CARD2 , CK-8 , CK8 , CYK8 , K2C8 , K8 , KO

Cellular Localization

Cytoplasm

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 8 antibody [N1C1] used in Western Blot (WB). GTX112975

GTX112975 WB Image

KO/KD

Wild-type (WT) and Krt8 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Krt8 antibody (GTX112975) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 8 antibody [N1C1] used in Western Blot (WB). GTX112975

GTX112975 WB Image

Sample (50 μg of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX112975 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 8 antibody [N1C1] used in Western Blot (WB). GTX112975

GTX112975 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] (GTX112975) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 8 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112975

GTX112975 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 8(GTX112975) antibody at 1:200 dilution.

Anti-Cytokeratin 8 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112975

GTX112975 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Cytokeratin 8(GTX112975) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 8 antibody [N1C1] used in Western Blot (WB). GTX112975

GTX112975 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] (GTX112975) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 8 antibody [N1C1] used in Western Blot (WB). GTX112975

GTX112975 WB Image

Whole zebrafish (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] (GTX112975) diluted at 1:700. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Kuo CH et al. Cell Biochem Funct 2020; (Epub) Biomarker discovery in highly invasive lung cancer cell through proteomics approaches.
Application : WB
Reactivity : Human
Yu HI et al. J Pharm Biomed Anal 2018; 160 : 344-350 Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells.
Application : WB
Reactivity : Human
Li JM et al. Environ Mol Mutagen 2013; 54 (6) : 429-49 Hyaluronic acid-dependent protection against UVB-damaged human corneal cells.
Application : WB
Reactivity : Human
Lu CH et al. Arch Biochem Biophys 2013; 529 (2) : 146-56 Proteomic analysis of retinopathy-related plasma biomarkers in diabetic patients.
Application : WB
Reactivity : Human
Cho CK et al. J Proteome Res 2010; 9 (7) : 3574-82 Amniotic fluid proteome analysis from Down syndrome pregnancies for biomarker discovery.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169