Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1N3] (GTX109489) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Cytokeratin 8(GTX109489) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Cytokeratin 8 antibody [N1N3] detects Cytokeratin 8 protein at cytoplasm and membrane in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Cytokeratin 8 antibody [N1N3] (GTX109489) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 8(GTX109489) antibody at 1:200 dilution.

Sample (30μg whole cell lysate)
A:MOLT4 (GTX27912)
B:Raji (GTX27908)
10% SDS PAGE
GTX109489 diluted at 1:1000

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody (GTX109489) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis (whole mount) of zebrafish embryo, using Cytokeratin 8 antibody [N1N3] (GTX109489) at 1:200 dilution.

Cytokeratin 8 antibody detects Cytokeratin 8 protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytokeratin 8 stained by Cytokeratin 8 antibody (GTX109489) diluted at 1:500.