Cytokeratin 8 antibody [N1N3]

Cat. No. GTX109489

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Wm
Reactivity	Human, Mouse, Zebrafish, Dog
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX109489 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Wm	Assay dependent

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Mouse liver , Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037299

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 8 , CARD2 , CK-8 , CK8 , CYK8 , K2C8 , K8 , KO

Cellular Localization

Cytoplasm

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq]

Database

Gene ID: 3856 KRT8
UniProt: P05787 KRT8

Research Area

DATA IMAGES

GTX109489 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX109489 diluted at 1:1000

GTX109489 WB Image

Sample (30μg whole cell lysate)
A:MOLT4 (GTX27912)
B:Raji (GTX27908)
10% SDS PAGE
GTX109489 diluted at 1:1000

GTX109489 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 8(GTX109489) antibody at 1:200 dilution.

GTX109489 IHC-P Image

Cytokeratin 8 antibody [N1N3] detects Cytokeratin 8 protein at cytoplasm and membrane in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Cytokeratin 8 antibody [N1N3] (GTX109489) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109489 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Cytokeratin 8(GTX109489) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109489 ICC/IF Image

Cytokeratin 8 antibody detects Cytokeratin 8 protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytokeratin 8 stained by Cytokeratin 8 antibody (GTX109489) diluted at 1:500.

GTX109489 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using Cytokeratin 8 antibody [N1N3] (GTX109489) at 1:200 dilution.

GTX109489 IHC-P Image

Cytokeratin 8 antibody detects Cytokeratin 8 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog skin.
Cytokeratin 8 stained by Cytokeratin 8 antibody (GTX109489) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109489 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody (GTX109489) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Takahashi Y et al. Clin Cancer Res 2012; 18 (22) : 6178-87 Establishment and characterization of novel cell lines from sinonasal undifferentiated carcinoma.

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REVIEW

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Application Reference

Takahashi Y et al. Clin Cancer Res 2012; 18 (22):6178-87 Establishment and characterization of novel cell lines from sinonasal undifferentiated carcinoma.

SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty

Cytokeratin 8 antibody [N1N3]

Cat. No. GTX109489

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX109489 WB Image

GTX109489 WB Image

GTX109489 ICC/IF Image

GTX109489 IHC-P Image

GTX109489 IHC-P Image

GTX109489 ICC/IF Image

GTX109489 IHC-Wm Image

GTX109489 IHC-P Image

GTX109489 WB Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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