GeneTex
United States (US)

Cytokeratin 8 antibody [RCK106]

Cytokeratin-8-antibody-RCK106_IHC_GTX29219-3_18121409_408.jpg
Cytokeratin-8-antibody-RCK106_IHC_GTX29219-1_18121409_668.jpg
Cytokeratin-8-antibody-RCK106_IHC_GTX29219-2_18121409_611.jpg

Cat No. GTX29219

Host Mouse
Clonality Monoclonal
Clone Name RCK106
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, Dot
Reactivity Human
APPLICATION

Application Note

Recommended dilutions: Dot: 1/100 - 1/1000. FACS: 1/100 - 1/200. ICC: Use at an assay dependent dilution. IHC-Fr: 1/100 - 1/200. WB: 1/100 - 1/1000.

Calculated MW

54 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts exclusively with cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.09% Sodium Azide Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Cytokeratins from the human bladder carcinoma cell line T24.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cytokeratin8, Cytokeratin 8, 3856, CYK8, 148060, KRT8, KO, P05787, K8, K2C8, CK8, CARD2

Background

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins, which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Database

Research Area

DATA IMAGES
Cytokeratin-8-antibody-RCK106_IHC_GTX29219-3_18121409_408.jpg

GTX29219 IHC-Fr Image

Immunohistochemistry on frozen section of human small intestine

Cytokeratin-8-antibody-RCK106_IHC_GTX29219-1_18121409_668.jpg

GTX29219 IHC-P Image

Immunohistochemistry on paraffin section of human colon

Cytokeratin-8-antibody-RCK106_IHC_GTX29219-2_18121409_611.jpg

GTX29219 IHC-Fr Image

Immunohistochemistry on frozen section of human kidney

Package List Price ($)
$ 329