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Cytokeratin 8 antibody [SPM192]

Anti-Cytokeratin 8 antibody [SPM192] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34668
Anti-Cytokeratin 8 antibody [SPM192] used in IHC (Paraffin sections) (IHC-P). GTX34668

Cat No. GTX34668

Host

Mouse

Clonality

Monoclonal

Clone Name

SPM192

Isotype

IgG1

Application

ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Specificity/Sensitivity

Epitope of this MAb is located between aa343-357.
We do not recommend use of this product for Rat.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Keratin preparation from a human carcinoma

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CARD2 , CK8 , CYK8 , K2C8 , K8 , KO , KRT8 , keratin 8 , Cytokeratin 8 , Keratin 8

Cellular Localization

Cytoplasm,Nucleus,Nucleoplasm,Nucleus matrix

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 8 antibody [SPM192] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34668

GTX34668 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX34668 Cytokeratin 8 antibody [SPM192].
Green : Primary antibody
Red : nucleus

Anti-Cytokeratin 8 antibody [SPM192] used in IHC (Paraffin sections) (IHC-P). GTX34668

GTX34668 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX34668 Cytokeratin 8 antibody [SPM192].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339