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Cytokeratin 8 antibody [SPM538]

Anti-Cytokeratin 8 antibody [SPM538] used in Flow cytometry (FACS). GTX34665
Anti-Cytokeratin 8 antibody [SPM538] used in IHC (Paraffin sections) (IHC-P). GTX34665
Anti-Cytokeratin 8 antibody [SPM538] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34665

Cat. No. GTX34665

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM538

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Rat, Zebrafish
Package
100 μg ($529)
See all Cytokeratin 8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Cytoskeleton preparation containing cytokeratin 8

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CARD2 , CK8 , CYK8 , K2C8 , K8 , KO , KRT8 , keratin 8 , Cytokeratin 8 , Keratin 8

Cellular Localization

Cytoplasm,Nucleus,Nucleoplasm,Nucleus matrix

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 8 antibody [SPM538] used in Flow cytometry (FACS). GTX34665

GTX34665 FACS Image

FACS analysis of trypsinized methanol-fixed HeLa cells using GTX34665 Cytokeratin 8 antibody [SPM538].
Blue : Primary antibody
Red : Isotype control

Anti-Cytokeratin 8 antibody [SPM538] used in IHC (Paraffin sections) (IHC-P). GTX34665

GTX34665 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX34665 Cytokeratin 8 antibody [SPM538].

Anti-Cytokeratin 8 antibody [SPM538] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34665

GTX34665 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX34665 Cytokeratin 8 antibody [SPM538].
Green : Primary antibody
Red : nucleus

REFERENCE

Application Reference

Liu J et al. Stem Cell Res 2020; 49 : 102010 Repair of acute liver damage with immune evasive hESC derived hepato-blasts.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529