Cytokeratin 8 antibody

Name: Cytokeratin 8 antibody
Brand: GeneTex
SKU: GTX110311
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Cytokeratin 8 antibody used in Western Blot (WB). GTX110311

Anti-Cytokeratin 8 antibody used in IHC (Whole mount) (IHC-Wm). GTX110311

Anti-Cytokeratin 8 antibody used in IHC (Paraffin sections) (IHC-P). GTX110311

Anti-Cytokeratin 8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110311

Cat. No. GTX110311

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Wm
Reactivity	Human, Mouse, Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all Cytokeratin 8 products

View product citations for antibody GTX110311 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Wm	Assay dependent

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Predict Reactivity

Rat, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165171

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 8 , CARD2 , CK-8 , CK8 , CYK8 , K2C8 , K8 , KO

Cellular Localization

Cytoplasm

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq]

Database

Gene ID: 3856 KRT8
UniProt: P05787 KRT8

Research Area

DATA IMAGES

GTX110311 WB Image

Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody (GTX110311) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110311 IHC-Wm Image

Cytokeratin 8 antibody detects Cytokeratin 8 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Cytokeratin 8 stained by Cytokeratin 8 antibody (GTX110311) diluted at 1:100.

GTX110311 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Gastric ca, using Cytokeratin 8(GTX110311) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX110311 WB Image

Sample (30 μg of whole cell lysate)
A: JC
10% SDS PAGE
GTX110311 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110311 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody (GTX110311) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110311 ICC/IF Image

Cytokeratin 8 antibody detects Cytokeratin 8 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytokeratin 8 stained by Cytokeratin 8 antibody (GTX110311) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX110311 IHC-Wm Image

The data was published in the journal Oncotarget in 2014. PMID: 25051368

REFERENCE

Application Reference

Chen CH et al. Oncotarget 2014; 5 (15) : 6300-11 Cdc6 cooperates with c-Myc to promote genome instability and epithelial to mesenchymal transition EMT in zebrafish.

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REVIEW