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Cytokeratin 8 antibody

Anti-Cytokeratin 8 antibody used in Western Blot (WB). GTX110311
Anti-Cytokeratin 8 antibody used in Western Blot (WB). GTX110311
Anti-Cytokeratin 8 antibody used in Western Blot (WB). GTX110311
Anti-Cytokeratin 8 antibody used in IHC (Paraffin sections) (IHC-P). GTX110311
Anti-Cytokeratin 8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110311

Cat No. GTX110311

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

Human, Mouse, Zebrafish
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HeLa , HepG2 , JC

Predict Reactivity

Rat, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165171

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

keratin 8 , CARD2 , CK-8 , CK8 , CYK8 , K2C8 , K8 , KO

Cellular Localization

Cytoplasm

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 8 antibody used in Western Blot (WB). GTX110311

GTX110311 WB Image

Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody (GTX110311) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 8 antibody used in Western Blot (WB). GTX110311

GTX110311 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody (GTX110311) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 8 antibody used in Western Blot (WB). GTX110311

GTX110311 WB Image

Sample (30 μg of whole cell lysate)
A: JC
10% SDS PAGE
GTX110311 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 8 antibody used in IHC (Paraffin sections) (IHC-P). GTX110311

GTX110311 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Gastric ca, using Cytokeratin 8(GTX110311) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110311

GTX110311 ICC/IF Image

Cytokeratin 8 antibody detects Cytokeratin 8 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytokeratin 8 stained by Cytokeratin 8 antibody (GTX110311) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

Cytokeratin 8 antibody Cat No. GTX110311

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Whole mount)(IHC-Wm) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2014.PMID: 25051368
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169