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Cytokeratin 8 antibody

Anti-Cytokeratin 8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32549
Anti-Cytokeratin 8 antibody used in IHC (Paraffin sections) (IHC-P). GTX32549
Anti-Cytokeratin 8 antibody used in Western Blot (WB). GTX32549
Anti-Cytokeratin 8 antibody used in Western Blot (WB). GTX32549
Anti-Cytokeratin 8 antibody used in IHC (Paraffin sections) (IHC-P). GTX32549

Cat No. GTX32549

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-483 of human KRT8 (NP_002264.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

keratin 8 , CARD2 , CK-8 , CK8 , CYK8 , K2C8 , K8 , KO

Cellular Localization

Cytoplasm,nucleoplasm

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32549

GTX32549 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX32549 Cytokeratin 8 antibody.
Blue : DAPI

Anti-Cytokeratin 8 antibody used in IHC (Paraffin sections) (IHC-P). GTX32549

GTX32549 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX32549 Cytokeratin 8 antibody.
Dilution : 1:100

Anti-Cytokeratin 8 antibody used in Western Blot (WB). GTX32549

GTX32549 WB Image

WB analysis of wild type and Cytokeratin 8 knockout cell lysate using GTX32549 Cytokeratin 8 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Cytokeratin 8 antibody used in Western Blot (WB). GTX32549

GTX32549 WB Image

WB analysis of various samples using GTX32549 Cytokeratin 8 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Cytokeratin 8 antibody used in IHC (Paraffin sections) (IHC-P). GTX32549

GTX32549 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX32549 Cytokeratin 8 antibody.
Dilution : 1:200

REFERENCE

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REVIEW

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Package List Price ($)
$ 339