GeneTex
United States (US)

Cytokeratin 8 antibody

KO/KD
GTX32549_20180530_ICCIF_18121410_132.jpg
GTX32549_20180530_IHC-P_18121410_361.jpg
GTX32549_20180822_WB_18121410_732.jpg
GTX32549_20180530_WB_18121410_522.jpg
GTX32549_20180530_IHC-P_1_18121410_931.jpg

Cat No. GTX32549

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

54 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-483 of human KRT8 (NP_002264.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Keratin 8,Card2,Ck-8,Ck8,Cyk8,K2C8,K8,Ko,Krt8

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES
GTX32549_20180530_ICCIF_18121410_132.jpg

GTX32549 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX32549 Cytokeratin 8 antibody.
Blue : DAPI

GTX32549_20180530_IHC-P_18121410_361.jpg

GTX32549 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX32549 Cytokeratin 8 antibody.
Dilution : 1:100

GTX32549_20180822_WB_18121410_732.jpg

GTX32549 WB Image

KO/KD

WB analysis of wild type and Cytokeratin 8 knockout cell lysate using GTX32549 Cytokeratin 8 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32549_20180530_WB_18121410_522.jpg

GTX32549 WB Image

WB analysis of various samples using GTX32549 Cytokeratin 8 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32549_20180530_IHC-P_1_18121410_931.jpg

GTX32549 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX32549 Cytokeratin 8 antibody.
Dilution : 1:200

Package List Price ($)
$ 329