GeneTex
  • Country / Location Selection

United States (US)

Cytokeratin 8 (phospho Ser74) antibody

Anti-Cytokeratin 8 (phospho Ser74) antibody used in Western Blot (WB). GTX50320
Anti-Cytokeratin 8 (phospho Ser74) antibody used in Western Blot (WB). GTX50320
Anti-Cytokeratin 8 (phospho Ser74) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50320

Cat No. GTX50320

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 74 (L-L-S(p)-P-L) derived from human Cytokeratin 8.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

keratin 8 , CARD2 , CK-8 , CK8 , CYK8 , K2C8 , K8 , KO

Cellular Localization

Cytoplasm,nucleoplasm

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 8 (phospho Ser74) antibody used in Western Blot (WB). GTX50320

GTX50320 WB Image

WB analysis of extracts from HepG2 cells untreated or treated with EGF using GTX50320 Cytokeratin 8 (phospho Ser74) antibody.

Anti-Cytokeratin 8 (phospho Ser74) antibody used in Western Blot (WB). GTX50320

GTX50320 WB Image

WB analysis of extracts from 293 cells treated with calf intestinal phosphatase (CIP) using GTX50320 Cytokeratin 8 (phospho Ser74) antibody.

Anti-Cytokeratin 8 (phospho Ser74) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50320

GTX50320 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50320 Cytokeratin 8 (phospho Ser74) antibody.

REFERENCE

There are currently no references for Cytokeratin 8 (phospho Ser74) antibody (GTX50320). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Cytokeratin 8 (phospho Ser74) antibody (GTX50320). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf