APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 2 μg/ml |
| 1:10 - 1:500 |
| 0.5 - 1 μg / 10⁶ cells |
| 1 - 2 μg/ml |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to the region of the Fas-induced cleavage site of human D4-GDI/RhoGDI2 was used as the immunogen.
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Rho GDP dissociation inhibitor beta , D4 , GDIA2 , GDID4 , LYGDI , Ly-GDI , RAP1GN1 , RhoGDI2
Cellular Localization
Cytoplasm, cytosol
Background
Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases (summary by Scherle et al., 1993 [PubMed 8356058]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
![Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Western Blot (WB). GTX11927](/templates/default/js/lightbox/images/loading.gif "Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Western Blot (WB). GTX11927")
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GTX11927 WB Image
WB analysis of HL-60 cells treated with 10 ng/ml anti-FAS mab (clone DX2) using GTX11927 D4 GDI (cleavage specific) antibody [97A1015].
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GTX11927 FACS Image
FACS (Intracellular staining) analysis of camptothecin-treated HL-60 cells using GTX11927 D4 GDI (cleavage specific) antibody [97A1015].
Red : Primary antibody
Green : isotype control
Shaded histogram : cell only
Antibody amount : 0.5 μg
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GTX11927 WB Image
WB analysis of 2 uM staurosporine treated Jurkat cell lysate using GTX11927 D4 GDI (cleavage specific) antibody [97A1015] (lane 4~6) or D4 GDI antibody (lane 1~3) as a control .
Dilution : 1 μg/ml
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GTX11927 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX11927 D4 GDI (cleavage specific) antibody [97A1015].
Top : Isotype control
Bottom : primary antibody
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GTX11927 FACS Image
FACS (Intracellular staining) analysis of 10⁶ untreated (left) and 3 hour staurosporine-treated (right) Jurkat cells using GTX11927 D4 GDI (cleavage specific) antibody [97A1015].
Red : Primary antibody
Green : isotype control
Antibody amount : 0.5 μg
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GTX11927 FACS Image
FACS (Intracellular staining) analysis of untreated (left) and staurosporine-treated (right) Ramos cells using GTX11927 D4 GDI (cleavage specific) antibody [97A1015].
Red : Primary antibody
Green : isotype control
Antibody amount : 0.5 μg
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REFERENCE
There are currently no references for D4 GDI (cleaved specific) antibody [97A1015] (GTX11927). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for D4 GDI (cleaved specific) antibody [97A1015] (GTX11927). Be the first to share your experience with this product.
![Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Western Blot (WB). GTX11927](/upload/website/prouct_img/small/GTX11927/GTX11927_894_WB_w_23060519_178.webp "Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Western Blot (WB). GTX11927")
![Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Flow cytometry (FACS). GTX11927](/upload/website/prouct_img/small/GTX11927/GTX11927_50_FACS_w_23060519_607.webp "Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Flow cytometry (FACS). GTX11927")
![Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Western Blot (WB). GTX11927](/upload/website/prouct_img/small/GTX11927/GTX11927_895_WB_w_23060519_902.webp "Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Western Blot (WB). GTX11927")
![Anti-D4 GDI (cleaved specific) antibody [97A1015] used in IHC (Paraffin sections) (IHC-P). GTX11927](/upload/website/prouct_img/small/GTX11927/GTX11927_379_IHC-P_w_23060519_406.webp "Anti-D4 GDI (cleaved specific) antibody [97A1015] used in IHC (Paraffin sections) (IHC-P). GTX11927")
![Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Flow cytometry (FACS). GTX11927](/upload/website/prouct_img/small/GTX11927/GTX11927_49_FACS_w_23060519_481.webp "Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Flow cytometry (FACS). GTX11927")
![Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Flow cytometry (FACS). GTX11927](/upload/website/prouct_img/small/GTX11927/GTX11927_52_FACS_w_23060519_782.webp "Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Flow cytometry (FACS). GTX11927")
![Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Western Blot (WB). GTX11927](/upload/website/prouct_img/normal/GTX11927/GTX11927_894_WB_w_23060519_178.webp "Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Western Blot (WB). GTX11927")
![Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Flow cytometry (FACS). GTX11927](/upload/website/prouct_img/normal/GTX11927/GTX11927_50_FACS_w_23060519_607.webp "Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Flow cytometry (FACS). GTX11927")
![Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Western Blot (WB). GTX11927](/upload/website/prouct_img/normal/GTX11927/GTX11927_895_WB_w_23060519_902.webp "Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Western Blot (WB). GTX11927")
![Anti-D4 GDI (cleaved specific) antibody [97A1015] used in IHC (Paraffin sections) (IHC-P). GTX11927](/upload/website/prouct_img/normal/GTX11927/GTX11927_379_IHC-P_w_23060519_406.webp "Anti-D4 GDI (cleaved specific) antibody [97A1015] used in IHC (Paraffin sections) (IHC-P). GTX11927")
![Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Flow cytometry (FACS). GTX11927](/upload/website/prouct_img/normal/GTX11927/GTX11927_49_FACS_w_23060519_481.webp "Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Flow cytometry (FACS). GTX11927")
![Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Flow cytometry (FACS). GTX11927](/upload/website/prouct_img/normal/GTX11927/GTX11927_52_FACS_w_23060519_782.webp "Anti-D4 GDI (cleaved specific) antibody [97A1015] used in Flow cytometry (FACS). GTX11927")